Dr. Joel Osuna Quintero
Ex-colaborador y/o ex-alumnoInvestigador Titular B Tiempo Completo
Total de publicaciones: 35
- Tipo
- Fecha
Artículo
1 - Arevalo-Salina,E.L., Osuna,J., Flores,H., Saab-Rincon,G. (2021). Engineering a calcium-dependent conformational change in Calbindin D(9k) by secondary elements replacement.
Archives of Biochemistry and Biophysics,
714,
109065 [corrigendum 109108].
Artículo
2 - Torres-Quintero, M.C., Gomez, I., Pacheco, S., Sanchez, J., Flores, H., Osuna, J., Mendoza, G., Soberon, M., Bravo, A. (2018). Engineering Bacillus thuringiensis Cyt1Aa toxin specificity from dipteran to lepidopteran toxicity.
Scientific Reports,
8 (1),
4989.
Artículo
3 - Carcamo, E., Roldan-Salgado, A., Osuna, J., Bello San-Martin, I., Yanez, J.A., Saab-Rincon, G., Viadiu, H., Gaytan, P. (2017). Spiked Genes: A Method to Introduce Random Point Nucleotide Mutations Evenly throughout an Entire Gene Using a Complete Set of Spiked Oligonucleotides for the Assembly.
ACS Omega,
2 (7),
3183-3191.
Artículo
4 - Rodriguez-Mejia, J.L., Roldan-Salgado, A., Osuna, J., Merino, E., Gaytan, P. (2017). A Codon Deletion at the Beginning of Green Fluorescent Protein Genes Enhances Protein Expression.
Journal of Molecular Microbiology and Biotechnology,
27 (1),
1-10.
Artículo
5 - Gonzalez-Bacerio,J., Osuna,J., Ponce,A., Fando,R., Figarella,K., Mendez,Y., Charli,J.L., Chavez,M.D. (2014). High-level expression in Escherichia coli, purification and kinetic characterization of Plasmodium falciparum M1-aminopeptidase.
Protein Expression and Purification,
104,
103-114.
Artículo
6 - Mendez,Y., Perez-Labrada,K., Gonzalez-Bacerio,J., Valdes,G., Chavez,M.D.A., Osuna,J., Charli,J.L., Pascual,I., Rivera,D.G. (2014). Combinatorial Multicomponent Access to Natural-Products-Inspired Peptidomimetics: Discovery of Selective Inhibitors of Microbial Metallo-aminopeptidases.
ChemMedChem,
9 (10),
2351-2359.
Artículo
7 - Osuna,J., Flores,H., Gaytan,P. (2012). A reporter system that discriminates EF-hand-sensor motifs from signal-modulators at the single-motif level.
FEBS Letters,
586 (19),
3398-3403.
Artículo
8 - Osuna,J., Flores,H., Saab-Rincon,G. (2012). The beta1 domain of protein G can replace the chorismate mutase domain of the T-protein.
FEBS Letters,
586 (4),
466-471.
Artículo
9 - Cote-Velez,A., Perez-Maldonado,A., Osuna,J., Barrera,B., Charli,J.L., Joseph-Bravo,P. (2011). Creb and Sp/Kruppel response elements cooperate to control rat TRH gene transcription in response to cAMP.
Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanisms,
1809 (3),
191-199.
Artículo
10 - Espino-Solis,G.P., Osuna-Quintero,J., Possani,L.D. (2008). Molecular cloning and characterization of the alphaX subunit from CD11c/CD18 horse integrin.
Veterinary Immunology and Immunopathology,
122 (3-4),
326-334.
Artículo
11 - Flores-Ramirez,G., Rivera,M., Morales-Pablos,A., Osuna,J., Soberon,X., Gaytan,P. (2007). The effect of amino acid deletions and substitutions in the longest loop of GFP.
BMC Chemical Biology,
7 (1),
1.
Artículo
12 - Vazquez-Limon,C., Vega-Badillo,J., Martinez,A., Espinosa-Molina,G., Gosset,G., Soberon,X., Lopez-Munguia,A., Osuna,J. (2007). Growth rate of a non-fermentative Escherichia coli strain is influenced by NAD(+) regeneration.
Biotechnology Letters,
29 (12),
1857-1863.
Artículo
13 - Chavez-Gutierrez,L., Matta-Camacho,E., Osuna,J., Horjales,E., Joseph-Bravo,P., Maigret,B., Charli,J.L. (2006). Homology modeling and site directed mutagenesis of pyroglutamyl peptidase II. Insights into omega versus aminopeptidase specificity in the M1 family.
Journal of Biological Chemistry,
281 (27),
18581-18590.
Artículo
14 - Monroy-Lagos,O., Soberon,X., Gaytan,P., Osuna,J. (2006). Improvement of an unusual twin-arginine transporter leader Peptide by a codon-based randomization approach.
Applied and Environmental Microbiology,
72 (5),
3797-3801.
Artículo
15 - Baez-Viveros,J.L., Osuna,J., Hernandez-Chavez,G., Soberon,X., Bolivar,F., Gosset,G. (2004). Metabolic engineering and protein directed evolution increase the yield of L-phenylalanine synthesized from glucose in Escherichia coli.
Biotechnology and Bioengineering,
87 (4),
516-524.
Artículo
16 - Flores,G., Soberon,X., Osuna,J. (2004). Production of a fully functional, permuted single-chain penicillin G acylase.
Protein Science,
13 (6),
1677-1683.
Artículo
17 - Osuna,J., Yanez,J., Soberon,X., Gaytan,P. (2004). Protein evolution by codon-based random deletions.
Nucleic Acids Research,
32 (17),
e136.
Artículo
18 - Yanez,J., Arguello,M., Osuna,J., Soberon,X., Gaytan,P. (2004). Combinatorial codon-based amino acid substitutions.
Nucleic Acids Research,
32 (20),
e158.
Artículo
19 - Gaytan,P., Osuna,J., Soberon,X. (2002). Novel ceftazidime-resistance beta-lactamases generated by a codon-based mutagenesis method and selection.
Nucleic Acids Research,
30 (16),
e84-e84.
Artículo
20 - Osuna,J., Perez-Blancas,A., Soberon,X. (2002). Improving a circularly permuted TEM-1 beta-lactamase by directed evolution.
Protein Engineering,
15 (6),
463-470.
Artículo
21 - Saab-Rincon,G., Juarez,V.R., Osuna,J., Sanchez,F., Soberon,X. (2001). Different strategies to recover the activity of monomeric triosephosphate isomerase by directed evolution.
Protein Engineering,
14 (3),
149-155.
Artículo
22 - Galindo,E., Lagunas,F., Osuna,J., Soberon,X., Garcia,J.L. (1998). A microbial biosensor for 6-aminopenicillanic acid.
Enzyme and Microbial Technology,
23 (5),
331-334.
Artículo
23 - Garcia,J.L., Nunez,C.J., Gonzalez,E.G., Osuna,J., Soberon,X., Galindo,E. (1998). Microbial sensor for new-generation cephalosporins based in a protein-engineered beta-lactamase.
Applied Biochemistry and Biotechnology,
73 (2-3),
243-256.
Artículo
24 - Osuna,J., Soberon,X., Morett,E. (1997). A proposed architecture for the central domain of the bacterial enhancer-binding proteins based on secondary structure prediction and fold recognition.
Protein Science,
6 (3),
543-555.
Revisión
25 - Hubbard,T., Tramontano,A., Barton,G., Jones,D., Sippl,M., Valencia,A., Lesk,A., Moult,J., Rost,B., Sander,C., Schneider,R., Lahm,A., Leplae,R., Buta,C., Eisenstein,M., Fjellstrom,O., Floeckner,H., Grossmann,J.G., Hansen,J., Citterich,M.H., Joergensen,F.S., Marchler-Bauer,A., Osuna,J., Park,J., Reinhardt,A., de-Pouplana,L.R., Rojo-Dominguez,A., Saudek,V., Sinclair,J., Sturrock,S., Venclovas,C., Vinals,C. (1996). Update on protein structure prediction: Results of the 1995 IRBM workshop .
Folding & Design,
1 (3),
R55-R63.
*
Artículo
26 - Flores,H., Osuna,J., Heitman,J., Soberon,X. (1995). Saturation mutagenesis of His114 of EcoRI reveals relaxed-specificity mutants.
Gene,
157 (1-2),
295-301.
Artículo
27 - Osuna,J., Viadiu,H., Fink,A.L., Soberon,X. (1995). Substitution of Asp for Asn at position 132 in the active site of TEM beta-lactamase. Activity toward different substrates and effects of neighboring residues.
Journal of Biological Chemistry,
270 (2),
775-780.
Artículo
28 - Viadiu,H., Osuna,J., Fink,A.L., Soberon,X. (1995). A new TEM beta-lactamase double mutant with broadened specificity reveals substrate-dependent functional interactions.
Journal of Biological Chemistry,
270 (2),
781-787.
Artículo
29 - Del Rio,G., Osuna,J., Soberon,X. (1994). Combinatorial libraries of proteins: analysis of efficiency of mutagenesis techniques.
Biotechniques,
17 (6),
1132-1139.
Revisión
30 - Osuna,J., Flores,H., Soberon,X. (1994). Microbial systems and directed evolution of protein activities.
Critical Reviews in Microbiology,
20 (2),
107-116.
Artículo
31 - Merino,E., Osuna,J., Bolivar,F., Soberon,X. (1992). A general, PCR-based method for single or combinatorial oligonucleotide-directed mutagenesis on pUC/M13 vectors.
Biotechniques,
12 (4),
508-510.
Artículo
Nacional
33 - Baez-Viveros,J.L., Osuna,J., Hernandez-Chavez,G., Soberon,X., Bolivar,F., Gosset,G. (2006). Metabolic via engineering for the production of aromatic substances in Escherichia coli.
Revista Latinoamericana de Microbiologia,
48 (2),
92.
34 - Osuna Quintero, Joel (1992). Analisis del sitio activo de enzimas mediante mutagenesis combinatoria .
Instituto de Biotecnologia,
Doctor en Investigacion Biomedica Basica,
UNAM.
35 - Osuna Quintero, Joel (1989). Aislamineto de un fragmento inmunogenico de toxina tetanica.
CEINGEBI,
Maestria en Investigacion Biomedica Basica,
UNAM.
Artículo
36 - Arevalo-Salina,E.L., Osuna,J., Flores,H., Saab-Rincon,G.. Engineering a calcium-dependent conformational change in Calbindin D(9k) by secondary elements replacement.
Archives of Biochemistry and Biophysics,
714,
109065 [corrigendum 109108].
Artículo
37 - Torres-Quintero, M.C., Gomez, I., Pacheco, S., Sanchez, J., Flores, H., Osuna, J., Mendoza, G., Soberon, M., Bravo, A.. Engineering Bacillus thuringiensis Cyt1Aa toxin specificity from dipteran to lepidopteran toxicity.
Scientific Reports,
8 (1),
4989.
Artículo
38 - Carcamo, E., Roldan-Salgado, A., Osuna, J., Bello San-Martin, I., Yanez, J.A., Saab-Rincon, G., Viadiu, H., Gaytan, P.. Spiked Genes: A Method to Introduce Random Point Nucleotide Mutations Evenly throughout an Entire Gene Using a Complete Set of Spiked Oligonucleotides for the Assembly.
ACS Omega,
2 (7),
3183-3191.
Artículo
39 - Rodriguez-Mejia, J.L., Roldan-Salgado, A., Osuna, J., Merino, E., Gaytan, P.. A Codon Deletion at the Beginning of Green Fluorescent Protein Genes Enhances Protein Expression.
Journal of Molecular Microbiology and Biotechnology,
27 (1),
1-10.
Artículo
40 - Gonzalez-Bacerio,J., Osuna,J., Ponce,A., Fando,R., Figarella,K., Mendez,Y., Charli,J.L., Chavez,M.D.. High-level expression in Escherichia coli, purification and kinetic characterization of Plasmodium falciparum M1-aminopeptidase.
Protein Expression and Purification,
104,
103-114.
Artículo
41 - Mendez,Y., Perez-Labrada,K., Gonzalez-Bacerio,J., Valdes,G., Chavez,M.D.A., Osuna,J., Charli,J.L., Pascual,I., Rivera,D.G.. Combinatorial Multicomponent Access to Natural-Products-Inspired Peptidomimetics: Discovery of Selective Inhibitors of Microbial Metallo-aminopeptidases.
ChemMedChem,
9 (10),
2351-2359.
Artículo
42 - Osuna,J., Flores,H., Gaytan,P.. A reporter system that discriminates EF-hand-sensor motifs from signal-modulators at the single-motif level.
FEBS Letters,
586 (19),
3398-3403.
Artículo
43 - Osuna,J., Flores,H., Saab-Rincon,G.. The beta1 domain of protein G can replace the chorismate mutase domain of the T-protein.
FEBS Letters,
586 (4),
466-471.
Artículo
44 - Cote-Velez,A., Perez-Maldonado,A., Osuna,J., Barrera,B., Charli,J.L., Joseph-Bravo,P.. Creb and Sp/Kruppel response elements cooperate to control rat TRH gene transcription in response to cAMP.
Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanisms,
1809 (3),
191-199.
Artículo
45 - Espino-Solis,G.P., Osuna-Quintero,J., Possani,L.D.. Molecular cloning and characterization of the alphaX subunit from CD11c/CD18 horse integrin.
Veterinary Immunology and Immunopathology,
122 (3-4),
326-334.
Artículo
46 - Flores-Ramirez,G., Rivera,M., Morales-Pablos,A., Osuna,J., Soberon,X., Gaytan,P.. The effect of amino acid deletions and substitutions in the longest loop of GFP.
BMC Chemical Biology,
7 (1),
1.
Artículo
47 - Vazquez-Limon,C., Vega-Badillo,J., Martinez,A., Espinosa-Molina,G., Gosset,G., Soberon,X., Lopez-Munguia,A., Osuna,J.. Growth rate of a non-fermentative Escherichia coli strain is influenced by NAD(+) regeneration.
Biotechnology Letters,
29 (12),
1857-1863.
Artículo
48 - Chavez-Gutierrez,L., Matta-Camacho,E., Osuna,J., Horjales,E., Joseph-Bravo,P., Maigret,B., Charli,J.L.. Homology modeling and site directed mutagenesis of pyroglutamyl peptidase II. Insights into omega versus aminopeptidase specificity in the M1 family.
Journal of Biological Chemistry,
281 (27),
18581-18590.
Artículo
49 - Monroy-Lagos,O., Soberon,X., Gaytan,P., Osuna,J.. Improvement of an unusual twin-arginine transporter leader Peptide by a codon-based randomization approach.
Applied and Environmental Microbiology,
72 (5),
3797-3801.
Nacional
50 - Baez-Viveros,J.L., Osuna,J., Hernandez-Chavez,G., Soberon,X., Bolivar,F., Gosset,G.. Metabolic via engineering for the production of aromatic substances in Escherichia coli.
Revista Latinoamericana de Microbiologia,
48 (2),
92.
Artículo
51 - Baez-Viveros,J.L., Osuna,J., Hernandez-Chavez,G., Soberon,X., Bolivar,F., Gosset,G.. Metabolic engineering and protein directed evolution increase the yield of L-phenylalanine synthesized from glucose in Escherichia coli.
Biotechnology and Bioengineering,
87 (4),
516-524.
Artículo
52 - Flores,G., Soberon,X., Osuna,J.. Production of a fully functional, permuted single-chain penicillin G acylase.
Protein Science,
13 (6),
1677-1683.
Artículo
53 - Osuna,J., Yanez,J., Soberon,X., Gaytan,P.. Protein evolution by codon-based random deletions.
Nucleic Acids Research,
32 (17),
e136.
Artículo
54 - Yanez,J., Arguello,M., Osuna,J., Soberon,X., Gaytan,P.. Combinatorial codon-based amino acid substitutions.
Nucleic Acids Research,
32 (20),
e158.
Artículo
55 - Gaytan,P., Osuna,J., Soberon,X.. Novel ceftazidime-resistance beta-lactamases generated by a codon-based mutagenesis method and selection.
Nucleic Acids Research,
30 (16),
e84-e84.
Artículo
56 - Osuna,J., Perez-Blancas,A., Soberon,X.. Improving a circularly permuted TEM-1 beta-lactamase by directed evolution.
Protein Engineering,
15 (6),
463-470.
Artículo
57 - Saab-Rincon,G., Juarez,V.R., Osuna,J., Sanchez,F., Soberon,X.. Different strategies to recover the activity of monomeric triosephosphate isomerase by directed evolution.
Protein Engineering,
14 (3),
149-155.
Artículo
58 - Galindo,E., Lagunas,F., Osuna,J., Soberon,X., Garcia,J.L.. A microbial biosensor for 6-aminopenicillanic acid.
Enzyme and Microbial Technology,
23 (5),
331-334.
Artículo
59 - Garcia,J.L., Nunez,C.J., Gonzalez,E.G., Osuna,J., Soberon,X., Galindo,E.. Microbial sensor for new-generation cephalosporins based in a protein-engineered beta-lactamase.
Applied Biochemistry and Biotechnology,
73 (2-3),
243-256.
Artículo
60 - Osuna,J., Soberon,X., Morett,E.. A proposed architecture for the central domain of the bacterial enhancer-binding proteins based on secondary structure prediction and fold recognition.
Protein Science,
6 (3),
543-555.
Revisión
61 - Hubbard,T., Tramontano,A., Barton,G., Jones,D., Sippl,M., Valencia,A., Lesk,A., Moult,J., Rost,B., Sander,C., Schneider,R., Lahm,A., Leplae,R., Buta,C., Eisenstein,M., Fjellstrom,O., Floeckner,H., Grossmann,J.G., Hansen,J., Citterich,M.H., Joergensen,F.S., Marchler-Bauer,A., Osuna,J., Park,J., Reinhardt,A., de-Pouplana,L.R., Rojo-Dominguez,A., Saudek,V., Sinclair,J., Sturrock,S., Venclovas,C., Vinals,C.. Update on protein structure prediction: Results of the 1995 IRBM workshop .
Folding & Design,
1 (3),
R55-R63.
*
Artículo
62 - Flores,H., Osuna,J., Heitman,J., Soberon,X.. Saturation mutagenesis of His114 of EcoRI reveals relaxed-specificity mutants.
Gene,
157 (1-2),
295-301.
Artículo
63 - Osuna,J., Viadiu,H., Fink,A.L., Soberon,X.. Substitution of Asp for Asn at position 132 in the active site of TEM beta-lactamase. Activity toward different substrates and effects of neighboring residues.
Journal of Biological Chemistry,
270 (2),
775-780.
Artículo
64 - Viadiu,H., Osuna,J., Fink,A.L., Soberon,X.. A new TEM beta-lactamase double mutant with broadened specificity reveals substrate-dependent functional interactions.
Journal of Biological Chemistry,
270 (2),
781-787.
Artículo
65 - Del Rio,G., Osuna,J., Soberon,X.. Combinatorial libraries of proteins: analysis of efficiency of mutagenesis techniques.
Biotechniques,
17 (6),
1132-1139.
Revisión
66 - Osuna,J., Flores,H., Soberon,X.. Microbial systems and directed evolution of protein activities.
Critical Reviews in Microbiology,
20 (2),
107-116.
Artículo
67 - Merino,E., Osuna,J., Bolivar,F., Soberon,X.. A general, PCR-based method for single or combinatorial oligonucleotide-directed mutagenesis on pUC/M13 vectors.
Biotechniques,
12 (4),
508-510.
68 - Osuna Quintero, Joel. Analisis del sitio activo de enzimas mediante mutagenesis combinatoria .
Instituto de Biotecnologia,
Doctor en Investigacion Biomedica Basica,
UNAM.
70 - Osuna Quintero, Joel. Aislamineto de un fragmento inmunogenico de toxina tetanica.
CEINGEBI,
Maestria en Investigacion Biomedica Basica,
UNAM.