Dr. Sabino Pacheco Guillen
Investigador Titular A Tiempo Completo
Microbiología Industrial utilizando proteinas bioinsecticidas
Número de registros: 5
- Activos
- Vencidos
- Estudio del sinergismo de toxinas vip3a y edición genética de sus potenciales receptores en spodoptera frugiperda, DIRECCIÓN GENERAL DE ASUNTOS DEL PERSONAL ACADÉMICO/UNIVERSIDAD NACIONAL AUTÓNOMA DE MÉXICO, 2024 - 2026
- Elucidando el papel funcional de múltiples receptores para las toxinas cry mosquitocidas producidas por bacillus thuringiensis, CONSEJO NACIONAL DE CIENCIA Y TECNOLOGÍA, 2020 - 2022
- Análisis genético/funcional de los trasportadores abc como moduladores de toxicidad de las proteínas cry de bacillus thuringiensis en aedes aegypti, DIRECCIÓN GENERAL DE ASUNTOS DEL PERSONAL ACADÉMICO/UNIVERSIDAD NACIONAL AUTÓNOMA DE MÉXICO, 2021 - 2023
- Estudio de la arquitectura de poros inducidos por las toxinas cry1a de bacillus thuringiensis en membranas utilizando fluorescencia, DIRECCIÓN GENERAL DE ASUNTOS DEL PERSONAL ACADÉMICO/UNIVERSIDAD NACIONAL AUTÓNOMA DE MÉXICO, 2018 - 2020
- Evolución dirigida de toxinas cry de bacillus thuringiensis como estrategia para rediseñar el reconocimiento toxina-receptor y contender con la resistencia de plagas agrícolas, DIRECCIÓN GENERAL DE ASUNTOS DEL PERSONAL ACADÉMICO/UNIVERSIDAD NACIONAL AUTÓNOMA DE MÉXICO, 2016 - 2018
Nivel I del SNII
- Activos
- Graduados
-
Doctorado
-
Maestría
-
Estancia Temporal
1 - Gallegos Hernandez, Adrian Salvador (2024). Analisis in vivo de los receptores apn1 y malp de las toxinas cry mediante edicion genetica por crispr-cas9 en aedes aegypti.
Instituto de Biotecnologia,
Maestria en Ciencias Bioquimicas,
UNAM.
Asesor: Pacheco Guillen, Sabino
2 - Guzmán Álvarez, María Elena (2024). Expresión recombinante y evaluación de la actividad insecticida de las toxinas vip3af y vip3ag de bacillus thuringiensis gr007.
Licenciatura en Ingenieria en Biotecnología,
Universidad Politecnica de Puebla.
*
Asesor: Pacheco Guilllen,Sabino
3 - Maldonado Tiro, Jesús Michel (2023). Detección de mutaciones en el genoma de Aedes aegypti generadas por CRISP-Cas9.
Ingeniero en Biotecnologia,
Universidad Politécnica de Puebla.
*
Asesor: Pacheco Guillen, Sabino
Total de publicaciones: 43
- Tipo
- Fecha
Artículo
4 - Lopez-Molina,S., Guerrero,A., Pacheco,S., Wang,Z., Zhang,J., Sanchez,J., Zavala,G., Soberon,M., Bravo,A. (2025). Cry11Aa toxin of Bacillus thuringiensis interactions with intracellular organelles in insect gut implicating actin depolymerization, massive endocytosis, and vesicle secretion.
International Journal of Biological Macromolecules,
314,
144350.
Artículo
5 - Pacheco,S., Chinas,M., Gomez,J.U., Pelaez-Aguilar,A.E., do Nascimento,N.A., Canton,P.E., Sanchez,J., Lopez-Molina,S., Gomez,I., Soberon,M., Bravo,A. (2025). ABC transporters knockout in Aedes aegypti induces upregulation of paralogous genes, avoiding resistance development to Bacillus thuringiensis Cry toxins.
PLoS ONE,
20 (7),
e0327221.
Artículo
6 - Pacheco,S., Gallegos,A.S., Pelaez-Aguilar,A.E., Sanchez,J., Gomez,I., Soberon,M., Bravo,A. (2024). CRISPR-Cas9 knockout of membrane-bound alkaline phosphatase or cadherin does not confer resistance to Cry toxins in Aedes aegypti.
PLoS Neglected Tropical Diseases,
18 (6),
e0012256.
Artículo
7 - Pacheco,S., Gomez,I., Soberon,M., Bravo,A. (2023). A major conformational change of N-terminal helices of Bacillus thuringiensis Cry1Ab insecticidal protein is necessary for membrane insertion and toxicity.
Febs Journal,
290 (10),
2692-2705.
Revisión
8 - Pacheco,S., Gomez,I., Pelaez-Aguilar,A.E., Verduzco-Rosas,L.A., Garcia-Suarez,R., do Nascimento,N.A., Rivera-Najera,L.Y., Canton,P.E., Soberon,M., Bravo,A. (2023). Structural changes upon membrane insertion of the insecticidal pore-forming toxins produced by Bacillus thuringiensis.
Frontiers in Insect Science,
3,
1188891.
Artículo
9 - Lopez-Molina,S., do Nascimento,N.A., Silva-Filha,M.H.N.L., Guerrero,A., Sanchez,J., Pacheco,S., Gill,S.S., Soberon,M., Bravo,A. (2021). In vivo nanoscale analysis of the dynamic synergistic interaction of Bacillus thuringiensis Cry11Aa and Cyt1Aa toxins in Aedes aegypti.
PLoS Pathogens,
17 (1),
e1009199.
Artículo
10 - Pacheco,S., Gomez,I., Chinas,M., Sanchez,J., Soberon,M., Bravo,A. (2021). Whole Genome Sequencing Analysis of Bacillus thuringiensis GR007 Reveals Multiple Pesticidal Protein Genes.
Frontiers in Microbiology,
12,
3268.
Artículo
11 - Sena da Silva,I.H., Gomez,I., Pacheco,S., Sanchez,J., Zhang,J., Luque Castellane,T.C., Aparecida Desiderio J., Soberon,M., Bravo,A., Polanczyk,R.A. (2021). Bacillus thuringiensis Cry1Ab domain III -16 is involved in binding to prohibitin which correlates with toxicity against Helicoverpa armigera (Lepidoptera: Noctuidae).
Applied and Environmental Microbiology,
87 (2),
e01930-20.
Artículo
12 - Chimal-Cazares,F., Hernandez-Martinez,G., Pacheco,S., Ares,M.A., Soria-Bustos,J., Sanchez-Gutierrez,M., Izquierdo-Vega,J.A., Ibarra,J.A., Gonzalez,Y.Merchand JA, Gorvel,J.P., Meresse,S., De la Cruz,M.A. (2020). Molecular Characterization of SehB, a Type II Antitoxin of Salmonella enterica Serotype Typhimurium: Amino Acid Residues Involved in DNA-Binding, Homodimerization, Toxin Interaction, and Virulence.
Frontiers in Microbiology,
11,
614.
Artículo
13 - do Nascimento,N.A., Torres-Quintero,M.C., Lopez-Molina,S., Pacheco,S., Romao,T.P., Pereira-Neves,A., Soberon,M., Bravo,A., Silva-Filha,M.H.N.L. (2020). Functional Bacillus thuringiensis Cyt1Aa is necessary to synergize Lysinibacillus sphaericus Binary toxin against Bin-resistant and refractory mosquito species.
Applied and Environmental Microbiology,
86 (7),
02770-19.
Artículo
14 - Onofre,J., Pacheco,S., Torres-Quintero,M.C., Gill,S.S., Soberon,M., Bravo,A. (2020). The Cyt1Aa toxin from Bacillus thuringiensis inserts into target membranes via different mechanisms in insects, red blood cells, and lipid liposomes.
Journal of Biological Chemistry,
295 (28),
9606-9617.
Artículo
15 - Pacheco,S., Quiliche,J.P.J., Gomez,I., Sanchez,J., Soberon,M., Bravo,A. (2020). Rearrangement of N-Terminal alpha-Helices of Bacillus thuringiensis Cry1Ab Toxin Essential for Oligomer Assembly and Toxicity.
Toxins (Basel),
12 (10),
E647.
Artículo
16 - Gomez, I., Rodriguez-Chamorro, D.E., Flores-Ramirez, G., Grande, R., Zuniga, F., Portugal, F.J., Sanchez, J., Pacheco, S., Bravo, A., Soberon, M. (2018). Spodoptera frugiperda (J. E. Smith) aminopeptidase N1 is functional receptor of Bacillus thuringiensis Cry1Ca toxin.
Applied and Environmental Microbiology,
84 (17),
UNSP e01089-18
.
Artículo
17 - Pacheco, S., Gomez, I., Sanchez, J., Garcia-Gomez, B.I., Czajkowsky, D.M., Zhang, J., Soberon, M., Bravo, A. (2018). Helix alpha-3 inter-molecular salt bridges and conformational changes are essential for toxicity of Bacillus thuringiensis 3D-Cry toxin family.
Scientific Reports,
8 (1),
10331.
Revisión
18 - Soberon, M., Portugal, L., Garcia-Gomez, B.I., Sanchez, J., Onofre, J., Gomez, I., Pacheco, S., Bravo, A. (2018). Cell lines as models for the study of Cry toxins from Bacillus thuringiensis.
Insect Biochemistry and Molecular Biology,
93,
66-78.
Artículo
19 - Torres-Quintero, M.C., Gomez, I., Pacheco, S., Sanchez, J., Flores, H., Osuna, J., Mendoza, G., Soberon, M., Bravo, A. (2018). Engineering Bacillus thuringiensis Cyt1Aa toxin specificity from dipteran to lepidopteran toxicity.
Scientific Reports,
8 (1),
4989.
Artículo
20 - Wang, Z., Fang, L., Zhou, Z., Pacheco, S., Gomez, I., Song, F., Soberon, M., Zhang, J., Bravo, A. (2018). Specific binding between Bacillus thuringiensis Cry9Aa and Vip3Aa toxins synergizes their toxicity against Asiatic rice borer (Chilo suppressalis).
Journal of Biological Chemistry,
293 (29),
11447-11458.
Artículo
21 - Ares, M.A., Rios-Sarabia, N., De la Cruz, M.A., Rivera-Gutierrez, S., Garcia-Morales, L., Leon-Solis, L., Espitia, C., Pacheco, S., Cerna-Cortes, J.F., Helguera-Repetto, C.A., Garcia, M.J., Gonzalez Y, Merchand J.A. (2017). The sigma factor SigD of Mycobacterium tuberculosis putatively enhances gene expression of the septum site determining protein under stressful environments.
New Microbiologica,
40 (3).
Artículo
22 - Ares, M.A., Fernandez-Vazquez, J.L., Pacheco, S., Martinez-Santos, V.I., Jarillo-Quijada, M.D., Torres, J., Alcantar-Curiel, M.D., Gonzalez Y Merchand J.A., De la Cruz, M.A. (2017). Additional regulatory activities of MrkH for the transcriptional expression of the Klebsiella pneumoniae mrk genes: Antagonist of H-NS and repressor.
PLoS ONE,
12 (3),
e0173285.
Artículo
23 - De la Cruz, M.A., Ruiz-Tagle, A., Ares, M.A., Pacheco, S., Yanez, J.A., Cedillo, L., Torres, J., Giron, J.A. (2017). The Expression of Longus type 4 pilus of enterotoxigenic Escherichia coli is regulated by LngR and LngS, and by H-NS, CpxR, and CRP global regulators.
Environmental Microbiology,
19 (5),
1761-1775.
Artículo; Actas Paper
24 - Onofre, J., Gaytan, M.O., Pena-Cardena, A., Garcia-Gomez, B.I., Pacheco, S., Gomez, I., Bravo, A., Soberon, M. (2017). Identification of Aminopeptidase-N2 as a Cry2Ab binding protein in Manduca sexta.
Peptides,
98,
93-98.
Artículo
25 - Pacheco, S., Gomez, I., Sanchez, J., Garcia-Gomez, B.I., Soberon, M., Bravo, A. (2017). Intra-molecular salt bridge in Bacillus thuringiensis Cry4Ba toxin is involved in the stability of helix alpha-3 that is needed for oligomerization and insecticidal activity.
Applied and Environmental Microbiology,
83 (20),
e01515-e01517.
Artículo
26 - Xiao, Y., Dai, Q., Hu, R., Pacheco, S., Yang, Y., Liang, G., Soberon, M., Bravo, A., Liu, K., Wu, K. (2017). A Single Point Mutation Resulting in Cadherin Mis-localization Underpins Resistance against Bacillus thuringiensis Toxin in Cotton Bollworm.
Journal of Biological Chemistry,
292 (7),
2933-2943.
Artículo
27 - Cardenas-Mondragon,M.G., Ares,M.A., Panunzi,L.G., Pacheco,S., Camorlinga-Ponce,M., Giron,J.A., Torres,J., De la Cruz,M.A. (2016). Transcriptional Profiling of Type II Toxin-Antitoxin Genes of Helicobacter pylori under Different Environmental Conditions: Identification of HP0967-HP0968 System.
Frontiers in Microbiology,
7,
1872.
Artículo
28 - Pacheco,S., Canton,E., Zuniga-Navarrete,F., Pecorari,F., Bravo,A., Soberon,M. (2015). Improvement and efficient display of Bacillus thuringiensis toxins on M13 phages and ribosomes.
AMB Express,
5 (1),
73.
Artículo
29 - Behar,G., Pacheco,S., Maillasson,M., Mouratou,B., Pecorari,F. (2014). Switching an anti-IgG binding site between archaeal extremophilic proteins results in Affitins with enhanced pH stability.
Journal of Biotechnology,
192 (Part A),
123-129.
*
Artículo
30 - Correa,A., Pacheco,S., Mechaly,A.E., Obal,G., Behar,G., Mouratou,B., Oppezzo,P., Alzari,P.M., Pecorari,F. (2014). Potent and specific inhibition of glycosidases by small artificial binding proteins (affitins).
PLoS ONE,
9 (5),
e97438.
*
Artículo
31 - Pacheco,S., Behar,G., Maillasson,M., Mouratou,B., Pecorari,F. (2014). Affinity transfer to the archaeal extremophilic Sac7d protein by insertion of a CDR.
Protein Engineering Design & Selection,
27 (10),
431-438.
*
Artículo
32 - Ares,M.A., Alcantar-Curiel,M.D., Jimenez-Galicia,C., Rios-Sarabia,N., Pacheco,S., De la Cruz,M.A. (2013). Antibiotic resistance of gram-negative bacilli isolated from pediatric patients with nosocomial bloodstream infections in a Mexican tertiary care hospital.
Chemotherapy,
59 (5),
361-368.
Artículo
33 - Cancino-Rodezno,A., Alexander,C., Villasenor,R., Pacheco,S., Porta,H., Pauchet,Y., Soberon,M., Gill,S.S., Bravo,A. (2010). The mitogen-activated protein kinase p38 is involved in insect defense against cry toxins from Bacillus thuringiensis.
Insect Biochemistry and Molecular Biology,
40 (1),
58-63 [corrigendum 43 (9) 898 2013 ].
Artículo
34 - Gomez,I., Arenas,I., Pacheco,S., Bravo,A., Soberon,M. (2010). New Insights into the Mode of Action of Cry1Ab Toxin Used in Transgenic Insect-resistant Crops.
Southwestern Entomologist,
35 (3),
387-390.
Artículo
35 - Pacheco,S., Gomez,I., Gill,S.S., Bravo,A., Soberon,M. (2009). Enhancement of insecticidal activity of Bacillus thuringiensis Cry1A toxins by fragments of a toxin-binding cadherin correlates with oligomer formation.
Peptides,
30 (3),
583-588.
Artículo
36 - Pacheco,S., Gomez,I., Arenas,I., Saab-Rincon,G., Rodriguez-Almazan,C., Gill,S.S., Bravo,A., Soberon,M. (2009). Domain II loop 3 of Bacillus thuringiensis Cry1Ab toxin is involved in a "ping-pong" binding mechanism with Manduca sexta aminopetidase-N and cadherin receptors.
Journal of Biological Chemistry,
284 (47),
32750-32757.
Artículo
37 - Rodriguez-Almazan,C., Zavala,L.E., Munoz-Garay,C., Jimenez-Juarez,N., Pacheco,S., Masson,L., Soberon,M., Bravo,A. (2009). Dominant negative mutants of Bacillus thuringiensis Cry1Ab toxin function as anti-toxins: demonstration of the role of oligomerization in toxicity.
PLoS ONE,
4 (5),
e5545 [Erratum 2013 8 (2)].
Artículo; Actas Paper
38 - Fernandez,L.E., Gomez,I., Pacheco,S., Arenas,I., Gill,S.S., Bravo,A., Soberon,M. (2008). Employing phage display to study the mode of action of Bacillus thuringiensis Cry toxins.
Peptides,
29 (2),
324-329.
Artículo
39 - Pacheco,S., Gomez,I., Sato,R., Bravo,A., Soberon,M. (2006). Functional display of Bacillus thuringiensis Cry1Ac toxin on T7 phage.
Journal of Invertebrate Pathology,
92 (1),
45-49.
Capítulo internacional
40 - Bravo,A., Pacheco,S., Gomez,I., Soberon,M. (2023). Mode of action of Bacillus thuringiensis Cry pesticidal proteins. Insecticidal Proteins and RNAi in the Control of Insects .
Advances in Insect Physiology,
55-92,
65,
Cambridge, Mass.: Academic Press Inc..
41 - Bravo, A., Pacheco, S., Gomez, I., Garcia-Gomez, B., Onofre, J., Soberon, M. (2017). Insecticidal Proteins from Bacillus thuringiensis and Their Mechanism of Action. Bacillus thuringiensis and Lysinibacillus sphaericus: Characterization and use in the field of biocontrol.
53-66,
Cham: Springer.
*
Internacional
42 - Bravo, A., Martinez-de-Castro, D. L., Sanchez-Quintana, J., Canton, P. E., Mendoza, G., Gomez, I., Pacheco, S., Garcia-Gomez, B. I., Onofre, J., Ocelotl, J., Soberon, M. (2015). Mechanism of action of Bacillus thuringiensis insecticidal toxins and their use in the control of insect pests. Comprehensive Sourcebook of Bacterial Protein Toxins.
858-873,
4a ed.
Internacional
43 - Soberon,M., Garcia-Gomez,B.I., Pacheco,S., Sanchez-Quintana,J., Tabashnik,B.E., Bravo,A. (2015). Countering pest resistance with genetically modified Bt toxins. Bt resistance-characterization and strategies for GM crops expressing Bacillus thuringiensis .
CABI Biotechnology Series Volume: 4,
150-161,
Oxford.
Internacional
44 - Pacheco,S., Soberon,M. (2012). Phage display: Fundamentals and applications. Tools to Understand Protein-Protein Interactions.
143,
Kerala: Research Signpost.
nacional divulgacion
45 - Gomez,I., Pacheco,S. (2022). La bacteria Bacillus thuringiensis como bioinsecticida en la agricultura actual.
Biotecnología en Movimiento. Revista de divulgación del Instituto de Biotecnología de la UNAM,
29.
46 - Pacheco Guillen, Sabino (2010). Papel del asa 3 del dominio II de las toxinas Cry1A's de Bacillus thuringiensis en el mecanismo de toxicidad : un blanco potencial para modificar el reconocimiento de sus receptores.
Instituto de Biotecnologia,
Doctorado en Ciencias Bioquimicas,
UNAM.
Asesor: Soberon Chavez, Mario
Artículo
47 - Lopez-Molina,S., Guerrero,A., Pacheco,S., Wang,Z., Zhang,J., Sanchez,J., Zavala,G., Soberon,M., Bravo,A.. Cry11Aa toxin of Bacillus thuringiensis interactions with intracellular organelles in insect gut implicating actin depolymerization, massive endocytosis, and vesicle secretion.
International Journal of Biological Macromolecules,
314,
144350.
Artículo
48 - Pacheco,S., Chinas,M., Gomez,J.U., Pelaez-Aguilar,A.E., do Nascimento,N.A., Canton,P.E., Sanchez,J., Lopez-Molina,S., Gomez,I., Soberon,M., Bravo,A.. ABC transporters knockout in Aedes aegypti induces upregulation of paralogous genes, avoiding resistance development to Bacillus thuringiensis Cry toxins.
PLoS ONE,
20 (7),
e0327221.
Artículo
49 - Pacheco,S., Gallegos,A.S., Pelaez-Aguilar,A.E., Sanchez,J., Gomez,I., Soberon,M., Bravo,A.. CRISPR-Cas9 knockout of membrane-bound alkaline phosphatase or cadherin does not confer resistance to Cry toxins in Aedes aegypti.
PLoS Neglected Tropical Diseases,
18 (6),
e0012256.
Artículo
50 - Pacheco,S., Gomez,I., Soberon,M., Bravo,A.. A major conformational change of N-terminal helices of Bacillus thuringiensis Cry1Ab insecticidal protein is necessary for membrane insertion and toxicity.
Febs Journal,
290 (10),
2692-2705.
Revisión
51 - Pacheco,S., Gomez,I., Pelaez-Aguilar,A.E., Verduzco-Rosas,L.A., Garcia-Suarez,R., do Nascimento,N.A., Rivera-Najera,L.Y., Canton,P.E., Soberon,M., Bravo,A.. Structural changes upon membrane insertion of the insecticidal pore-forming toxins produced by Bacillus thuringiensis.
Frontiers in Insect Science,
3,
1188891.
Capítulo internacional
52 - Bravo,A., Pacheco,S., Gomez,I., Soberon,M.. Mode of action of Bacillus thuringiensis Cry pesticidal proteins. Insecticidal Proteins and RNAi in the Control of Insects .
Advances in Insect Physiology,
55-92,
65,
Cambridge, Mass.: Academic Press Inc..
nacional divulgacion
53 - Gomez,I., Pacheco,S.. La bacteria Bacillus thuringiensis como bioinsecticida en la agricultura actual.
Biotecnología en Movimiento. Revista de divulgación del Instituto de Biotecnología de la UNAM,
29.
Artículo
54 - Lopez-Molina,S., do Nascimento,N.A., Silva-Filha,M.H.N.L., Guerrero,A., Sanchez,J., Pacheco,S., Gill,S.S., Soberon,M., Bravo,A.. In vivo nanoscale analysis of the dynamic synergistic interaction of Bacillus thuringiensis Cry11Aa and Cyt1Aa toxins in Aedes aegypti.
PLoS Pathogens,
17 (1),
e1009199.
Artículo
55 - Pacheco,S., Gomez,I., Chinas,M., Sanchez,J., Soberon,M., Bravo,A.. Whole Genome Sequencing Analysis of Bacillus thuringiensis GR007 Reveals Multiple Pesticidal Protein Genes.
Frontiers in Microbiology,
12,
3268.
Artículo
56 - Sena da Silva,I.H., Gomez,I., Pacheco,S., Sanchez,J., Zhang,J., Luque Castellane,T.C., Aparecida Desiderio J., Soberon,M., Bravo,A., Polanczyk,R.A.. Bacillus thuringiensis Cry1Ab domain III -16 is involved in binding to prohibitin which correlates with toxicity against Helicoverpa armigera (Lepidoptera: Noctuidae).
Applied and Environmental Microbiology,
87 (2),
e01930-20.
Artículo
57 - Chimal-Cazares,F., Hernandez-Martinez,G., Pacheco,S., Ares,M.A., Soria-Bustos,J., Sanchez-Gutierrez,M., Izquierdo-Vega,J.A., Ibarra,J.A., Gonzalez,Y.Merchand JA, Gorvel,J.P., Meresse,S., De la Cruz,M.A.. Molecular Characterization of SehB, a Type II Antitoxin of Salmonella enterica Serotype Typhimurium: Amino Acid Residues Involved in DNA-Binding, Homodimerization, Toxin Interaction, and Virulence.
Frontiers in Microbiology,
11,
614.
Artículo
58 - do Nascimento,N.A., Torres-Quintero,M.C., Lopez-Molina,S., Pacheco,S., Romao,T.P., Pereira-Neves,A., Soberon,M., Bravo,A., Silva-Filha,M.H.N.L.. Functional Bacillus thuringiensis Cyt1Aa is necessary to synergize Lysinibacillus sphaericus Binary toxin against Bin-resistant and refractory mosquito species.
Applied and Environmental Microbiology,
86 (7),
02770-19.
Artículo
59 - Onofre,J., Pacheco,S., Torres-Quintero,M.C., Gill,S.S., Soberon,M., Bravo,A.. The Cyt1Aa toxin from Bacillus thuringiensis inserts into target membranes via different mechanisms in insects, red blood cells, and lipid liposomes.
Journal of Biological Chemistry,
295 (28),
9606-9617.
Artículo
60 - Pacheco,S., Quiliche,J.P.J., Gomez,I., Sanchez,J., Soberon,M., Bravo,A.. Rearrangement of N-Terminal alpha-Helices of Bacillus thuringiensis Cry1Ab Toxin Essential for Oligomer Assembly and Toxicity.
Toxins (Basel),
12 (10),
E647.
Artículo
61 - Gomez, I., Rodriguez-Chamorro, D.E., Flores-Ramirez, G., Grande, R., Zuniga, F., Portugal, F.J., Sanchez, J., Pacheco, S., Bravo, A., Soberon, M.. Spodoptera frugiperda (J. E. Smith) aminopeptidase N1 is functional receptor of Bacillus thuringiensis Cry1Ca toxin.
Applied and Environmental Microbiology,
84 (17),
UNSP e01089-18
.
Artículo
62 - Pacheco, S., Gomez, I., Sanchez, J., Garcia-Gomez, B.I., Czajkowsky, D.M., Zhang, J., Soberon, M., Bravo, A.. Helix alpha-3 inter-molecular salt bridges and conformational changes are essential for toxicity of Bacillus thuringiensis 3D-Cry toxin family.
Scientific Reports,
8 (1),
10331.
Revisión
63 - Soberon, M., Portugal, L., Garcia-Gomez, B.I., Sanchez, J., Onofre, J., Gomez, I., Pacheco, S., Bravo, A.. Cell lines as models for the study of Cry toxins from Bacillus thuringiensis.
Insect Biochemistry and Molecular Biology,
93,
66-78.
Artículo
64 - Torres-Quintero, M.C., Gomez, I., Pacheco, S., Sanchez, J., Flores, H., Osuna, J., Mendoza, G., Soberon, M., Bravo, A.. Engineering Bacillus thuringiensis Cyt1Aa toxin specificity from dipteran to lepidopteran toxicity.
Scientific Reports,
8 (1),
4989.
Artículo
65 - Wang, Z., Fang, L., Zhou, Z., Pacheco, S., Gomez, I., Song, F., Soberon, M., Zhang, J., Bravo, A.. Specific binding between Bacillus thuringiensis Cry9Aa and Vip3Aa toxins synergizes their toxicity against Asiatic rice borer (Chilo suppressalis).
Journal of Biological Chemistry,
293 (29),
11447-11458.
Artículo
66 - Ares, M.A., Rios-Sarabia, N., De la Cruz, M.A., Rivera-Gutierrez, S., Garcia-Morales, L., Leon-Solis, L., Espitia, C., Pacheco, S., Cerna-Cortes, J.F., Helguera-Repetto, C.A., Garcia, M.J., Gonzalez Y, Merchand J.A.. The sigma factor SigD of Mycobacterium tuberculosis putatively enhances gene expression of the septum site determining protein under stressful environments.
New Microbiologica,
40 (3).
Artículo
67 - Ares, M.A., Fernandez-Vazquez, J.L., Pacheco, S., Martinez-Santos, V.I., Jarillo-Quijada, M.D., Torres, J., Alcantar-Curiel, M.D., Gonzalez Y Merchand J.A., De la Cruz, M.A.. Additional regulatory activities of MrkH for the transcriptional expression of the Klebsiella pneumoniae mrk genes: Antagonist of H-NS and repressor.
PLoS ONE,
12 (3),
e0173285.
Artículo
68 - De la Cruz, M.A., Ruiz-Tagle, A., Ares, M.A., Pacheco, S., Yanez, J.A., Cedillo, L., Torres, J., Giron, J.A.. The Expression of Longus type 4 pilus of enterotoxigenic Escherichia coli is regulated by LngR and LngS, and by H-NS, CpxR, and CRP global regulators.
Environmental Microbiology,
19 (5),
1761-1775.
Artículo; Actas Paper
69 - Onofre, J., Gaytan, M.O., Pena-Cardena, A., Garcia-Gomez, B.I., Pacheco, S., Gomez, I., Bravo, A., Soberon, M.. Identification of Aminopeptidase-N2 as a Cry2Ab binding protein in Manduca sexta.
Peptides,
98,
93-98.
Artículo
70 - Pacheco, S., Gomez, I., Sanchez, J., Garcia-Gomez, B.I., Soberon, M., Bravo, A.. Intra-molecular salt bridge in Bacillus thuringiensis Cry4Ba toxin is involved in the stability of helix alpha-3 that is needed for oligomerization and insecticidal activity.
Applied and Environmental Microbiology,
83 (20),
e01515-e01517.
Artículo
71 - Xiao, Y., Dai, Q., Hu, R., Pacheco, S., Yang, Y., Liang, G., Soberon, M., Bravo, A., Liu, K., Wu, K.. A Single Point Mutation Resulting in Cadherin Mis-localization Underpins Resistance against Bacillus thuringiensis Toxin in Cotton Bollworm.
Journal of Biological Chemistry,
292 (7),
2933-2943.
72 - Bravo, A., Pacheco, S., Gomez, I., Garcia-Gomez, B., Onofre, J., Soberon, M.. Insecticidal Proteins from Bacillus thuringiensis and Their Mechanism of Action. Bacillus thuringiensis and Lysinibacillus sphaericus: Characterization and use in the field of biocontrol.
53-66,
Cham: Springer.
*
Artículo
73 - Cardenas-Mondragon,M.G., Ares,M.A., Panunzi,L.G., Pacheco,S., Camorlinga-Ponce,M., Giron,J.A., Torres,J., De la Cruz,M.A.. Transcriptional Profiling of Type II Toxin-Antitoxin Genes of Helicobacter pylori under Different Environmental Conditions: Identification of HP0967-HP0968 System.
Frontiers in Microbiology,
7,
1872.
Artículo
74 - Pacheco,S., Canton,E., Zuniga-Navarrete,F., Pecorari,F., Bravo,A., Soberon,M.. Improvement and efficient display of Bacillus thuringiensis toxins on M13 phages and ribosomes.
AMB Express,
5 (1),
73.
Internacional
75 - Bravo, A., Martinez-de-Castro, D. L., Sanchez-Quintana, J., Canton, P. E., Mendoza, G., Gomez, I., Pacheco, S., Garcia-Gomez, B. I., Onofre, J., Ocelotl, J., Soberon, M.. Mechanism of action of Bacillus thuringiensis insecticidal toxins and their use in the control of insect pests. Comprehensive Sourcebook of Bacterial Protein Toxins.
858-873,
4a ed.
Internacional
76 - Soberon,M., Garcia-Gomez,B.I., Pacheco,S., Sanchez-Quintana,J., Tabashnik,B.E., Bravo,A.. Countering pest resistance with genetically modified Bt toxins. Bt resistance-characterization and strategies for GM crops expressing Bacillus thuringiensis .
CABI Biotechnology Series Volume: 4,
150-161,
Oxford.
Artículo
77 - Behar,G., Pacheco,S., Maillasson,M., Mouratou,B., Pecorari,F.. Switching an anti-IgG binding site between archaeal extremophilic proteins results in Affitins with enhanced pH stability.
Journal of Biotechnology,
192 (Part A),
123-129.
*
Artículo
78 - Correa,A., Pacheco,S., Mechaly,A.E., Obal,G., Behar,G., Mouratou,B., Oppezzo,P., Alzari,P.M., Pecorari,F.. Potent and specific inhibition of glycosidases by small artificial binding proteins (affitins).
PLoS ONE,
9 (5),
e97438.
*
Artículo
79 - Pacheco,S., Behar,G., Maillasson,M., Mouratou,B., Pecorari,F.. Affinity transfer to the archaeal extremophilic Sac7d protein by insertion of a CDR.
Protein Engineering Design & Selection,
27 (10),
431-438.
*
Artículo
80 - Ares,M.A., Alcantar-Curiel,M.D., Jimenez-Galicia,C., Rios-Sarabia,N., Pacheco,S., De la Cruz,M.A.. Antibiotic resistance of gram-negative bacilli isolated from pediatric patients with nosocomial bloodstream infections in a Mexican tertiary care hospital.
Chemotherapy,
59 (5),
361-368.
Internacional
81 - Pacheco,S., Soberon,M.. Phage display: Fundamentals and applications. Tools to Understand Protein-Protein Interactions.
143,
Kerala: Research Signpost.
Artículo
82 - Cancino-Rodezno,A., Alexander,C., Villasenor,R., Pacheco,S., Porta,H., Pauchet,Y., Soberon,M., Gill,S.S., Bravo,A.. The mitogen-activated protein kinase p38 is involved in insect defense against cry toxins from Bacillus thuringiensis.
Insect Biochemistry and Molecular Biology,
40 (1),
58-63 [corrigendum 43 (9) 898 2013 ].
Artículo
83 - Gomez,I., Arenas,I., Pacheco,S., Bravo,A., Soberon,M.. New Insights into the Mode of Action of Cry1Ab Toxin Used in Transgenic Insect-resistant Crops.
Southwestern Entomologist,
35 (3),
387-390.
84 - Pacheco Guillen, Sabino. Papel del asa 3 del dominio II de las toxinas Cry1A's de Bacillus thuringiensis en el mecanismo de toxicidad : un blanco potencial para modificar el reconocimiento de sus receptores.
Instituto de Biotecnologia,
Doctorado en Ciencias Bioquimicas,
UNAM.
Asesor: Soberon Chavez, Mario
Artículo
85 - Pacheco,S., Gomez,I., Gill,S.S., Bravo,A., Soberon,M.. Enhancement of insecticidal activity of Bacillus thuringiensis Cry1A toxins by fragments of a toxin-binding cadherin correlates with oligomer formation.
Peptides,
30 (3),
583-588.
Artículo
86 - Pacheco,S., Gomez,I., Arenas,I., Saab-Rincon,G., Rodriguez-Almazan,C., Gill,S.S., Bravo,A., Soberon,M.. Domain II loop 3 of Bacillus thuringiensis Cry1Ab toxin is involved in a "ping-pong" binding mechanism with Manduca sexta aminopetidase-N and cadherin receptors.
Journal of Biological Chemistry,
284 (47),
32750-32757.
Artículo
87 - Rodriguez-Almazan,C., Zavala,L.E., Munoz-Garay,C., Jimenez-Juarez,N., Pacheco,S., Masson,L., Soberon,M., Bravo,A.. Dominant negative mutants of Bacillus thuringiensis Cry1Ab toxin function as anti-toxins: demonstration of the role of oligomerization in toxicity.
PLoS ONE,
4 (5),
e5545 [Erratum 2013 8 (2)].
Artículo; Actas Paper
88 - Fernandez,L.E., Gomez,I., Pacheco,S., Arenas,I., Gill,S.S., Bravo,A., Soberon,M.. Employing phage display to study the mode of action of Bacillus thuringiensis Cry toxins.
Peptides,
29 (2),
324-329.
Artículo
89 - Pacheco,S., Gomez,I., Sato,R., Bravo,A., Soberon,M.. Functional display of Bacillus thuringiensis Cry1Ac toxin on T7 phage.
Journal of Invertebrate Pathology,
92 (1),
45-49.