Dr. Sabino Pacheco Guillen
Investigador Titular A Tiempo Completo
Microbiología Industrial utilizando proteinas bioinsecticidas
Número de registros: 6
- Activos
- Vencidos
- Edición genética a gran escala mediada por crispr-cas9 para la búsqueda de factores relacionados con la citotoxicidad de las toxinas insecticidas de bacillus thuringiensis, Secretaria de Ciencia, Humanidades, tecnología e Innovación , 2025 - 1900
- Estudio del sinergismo de toxinas vip3a y edición genética de sus potenciales receptores en spodoptera frugiperda, DIRECCIÓN GENERAL DE ASUNTOS DEL PERSONAL ACADÉMICO/UNIVERSIDAD NACIONAL AUTÓNOMA DE MÉXICO, 2024 - 2026
- Análisis genético/funcional de los trasportadores abc como moduladores de toxicidad de las proteínas cry de bacillus thuringiensis en aedes aegypti, DIRECCIÓN GENERAL DE ASUNTOS DEL PERSONAL ACADÉMICO/UNIVERSIDAD NACIONAL AUTÓNOMA DE MÉXICO, 2021 - 2023
- Elucidando el papel funcional de múltiples receptores para las toxinas cry mosquitocidas producidas por bacillus thuringiensis, CONSEJO NACIONAL DE CIENCIA Y TECNOLOGÍA, 2020 - 2022
- Estudio de la arquitectura de poros inducidos por las toxinas cry1a de bacillus thuringiensis en membranas utilizando fluorescencia, DIRECCIÓN GENERAL DE ASUNTOS DEL PERSONAL ACADÉMICO/UNIVERSIDAD NACIONAL AUTÓNOMA DE MÉXICO, 2018 - 2020
- Evolución dirigida de toxinas cry de bacillus thuringiensis como estrategia para rediseñar el reconocimiento toxina-receptor y contender con la resistencia de plagas agrícolas, DIRECCIÓN GENERAL DE ASUNTOS DEL PERSONAL ACADÉMICO/UNIVERSIDAD NACIONAL AUTÓNOMA DE MÉXICO, 2016 - 2018
Nivel I del SNII
- Activos
- Graduados
-
Estancia Temporal
-
Doctorado (UNAM u Otros)
-
Maestría (UNAM u Otros)
1 - Gallegos Hernandez, Adrian Salvador (2024). Analisis in vivo de los receptores apn1 y malp de las toxinas cry mediante edicion genetica por crispr-cas9 en aedes aegypti.
Instituto de Biotecnologia,
Maestria en Ciencias Bioquimicas,
UNAM.
Asesor: Pacheco Guillen, Sabino
2 - Guzmán Álvarez, María Elena (2024). Expresión recombinante y evaluación de la actividad insecticida de las toxinas vip3af y vip3ag de bacillus thuringiensis gr007.
Licenciatura en Ingenieria en Biotecnología,
Universidad Politecnica de Puebla.
*
Asesor: Pacheco Guilllen,Sabino
3 - Maldonado Tiro, Jesús Michel (2023). Detección de mutaciones en el genoma de Aedes aegypti generadas por CRISP-Cas9.
Ingeniero en Biotecnologia,
Universidad Politécnica de Puebla.
*
Asesor: Pacheco Guillen, Sabino
4 - Gonzaga Perez, Ivan (2022). Obtención de una población homocigota de Aedes aegypti con mutaciones en el gen del receptor caderina.
Facultad de Ciencias Biológicas,
Licenciaure en Biología,
UAEM.
*
Asesor: Pacheco Guillen, Sabino
* Indica publicación con otra institución de adscripción
Total de publicaciones: 47
- Tipo
- Fecha
Artículo
5 - do Nascimento,N.A., Torres-Quintero,M.C., Garcia-Gomez,B.I., Gomez,I., Pacheco,S., Bravo,A., Soberon,M. (2026). Engineering Bacillus thuringiensis Cyt1Aa to function as a surrogate receptor of Cry1A lepidopteran insecticidal proteins.
Pesticide Biochemistry And Physiology,
219 (April),
106970,
[Correction 107201 2026].
Artículo
6 - Gomez,I., Garcia-Gomez,B.I., do Nascimento,N.A., Infante,O., Canton,P.E., Pacheco,S., Pelaez-Aguilar,A.E., Sanchez,J., Soberon,M., Bravo,A. (2026). Regulatory diversity in Bacillus thuringiensis cry genes reveals flexible evolutionary strategies for in vivo toxin expression.
PLoS Pathogens,
22 (3),
e1014017.
Artículo
7 - Quintana,A.J., Garcia-Suarez,R., Prieto,A., Sanchez,J., Gomez,I., Verduzco-Rosas,L.A., doNascimento,N., Lopez-Molina,S., Zhang,J., Soberon,M., Bravo,A., Pacheco,S. (2026). Genome editing of ABCB6 Transporter Confers Resistance to Cypermethrin in the Major Pest of Corn, Spodoptera frugiperda.
Insect Biochemistry and Molecular Biology,
190 (May),
104537.
Artículo
8 - Lopez-Molina,S., Guerrero,A., Pacheco,S., Wang,Z., Zhang,J., Sanchez,J., Zavala,G., Soberon,M., Bravo,A. (2025). Cry11Aa toxin of Bacillus thuringiensis interactions with intracellular organelles in insect gut implicating actin depolymerization, massive endocytosis, and vesicle secretion.
International Journal of Biological Macromolecules,
314,
144350.
Artículo
9 - Pacheco,S., Chinas,M., Gomez,J.U., Pelaez-Aguilar,A.E., do Nascimento,N.A., Canton,P.E., Sanchez,J., Lopez-Molina,S., Gomez,I., Soberon,M., Bravo,A. (2025). ABC transporters knockout in Aedes aegypti induces upregulation of paralogous genes, avoiding resistance development to Bacillus thuringiensis Cry toxins.
PLoS ONE,
20 (7),
e0327221.
Artículo
10 - Pacheco,S., Gallegos,A.S., Pelaez-Aguilar,A.E., Sanchez,J., Gomez,I., Soberon,M., Bravo,A. (2024). CRISPR-Cas9 knockout of membrane-bound alkaline phosphatase or cadherin does not confer resistance to Cry toxins in Aedes aegypti.
PLoS Neglected Tropical Diseases,
18 (6),
e0012256.
Revisión
11 - Pacheco,S., Gomez,I., Pelaez-Aguilar,A.E., Verduzco-Rosas,L.A., Garcia-Suarez,R., do Nascimento,N.A., Rivera-Najera,L.Y., Canton,P.E., Soberon,M., Bravo,A. (2023). Structural changes upon membrane insertion of the insecticidal pore-forming toxins produced by Bacillus thuringiensis.
Frontiers in Insect Science,
3,
1188891.
Artículo
12 - Pacheco,S., Gomez,I., Soberon,M., Bravo,A. (2023). A major conformational change of N-terminal helices of Bacillus thuringiensis Cry1Ab insecticidal protein is necessary for membrane insertion and toxicity.
Febs Journal,
290 (10),
2692-2705.
Artículo
13 - Lopez-Molina,S., do Nascimento,N.A., Silva-Filha,M.H.N.L., Guerrero,A., Sanchez,J., Pacheco,S., Gill,S.S., Soberon,M., Bravo,A. (2021). In vivo nanoscale analysis of the dynamic synergistic interaction of Bacillus thuringiensis Cry11Aa and Cyt1Aa toxins in Aedes aegypti.
PLoS Pathogens,
17 (1),
e1009199.
Artículo
14 - Pacheco,S., Gomez,I., Chinas,M., Sanchez,J., Soberon,M., Bravo,A. (2021). Whole Genome Sequencing Analysis of Bacillus thuringiensis GR007 Reveals Multiple Pesticidal Protein Genes.
Frontiers in Microbiology,
12,
3268.
Artículo
15 - Sena da Silva,I.H., Gomez,I., Pacheco,S., Sanchez,J., Zhang,J., Luque Castellane,T.C., Aparecida Desiderio J., Soberon,M., Bravo,A., Polanczyk,R.A. (2021). Bacillus thuringiensis Cry1Ab domain III -16 is involved in binding to prohibitin which correlates with toxicity against Helicoverpa armigera (Lepidoptera: Noctuidae).
Applied and Environmental Microbiology,
87 (2),
e01930-20.
Artículo
16 - Chimal-Cazares,F., Hernandez-Martinez,G., Pacheco,S., Ares,M.A., Soria-Bustos,J., Sanchez-Gutierrez,M., Izquierdo-Vega,J.A., Ibarra,J.A., Gonzalez,Y.Merchand JA, Gorvel,J.P., Meresse,S., De la Cruz,M.A. (2020). Molecular Characterization of SehB, a Type II Antitoxin of Salmonella enterica Serotype Typhimurium: Amino Acid Residues Involved in DNA-Binding, Homodimerization, Toxin Interaction, and Virulence.
Frontiers in Microbiology,
11,
614.
Artículo
17 - do Nascimento,N.A., Torres-Quintero,M.C., Lopez-Molina,S., Pacheco,S., Romao,T.P., Pereira-Neves,A., Soberon,M., Bravo,A., Silva-Filha,M.H.N.L. (2020). Functional Bacillus thuringiensis Cyt1Aa is necessary to synergize Lysinibacillus sphaericus Binary toxin against Bin-resistant and refractory mosquito species.
Applied and Environmental Microbiology,
86 (7),
02770-19.
Artículo
18 - Onofre,J., Pacheco,S., Torres-Quintero,M.C., Gill,S.S., Soberon,M., Bravo,A. (2020). The Cyt1Aa toxin from Bacillus thuringiensis inserts into target membranes via different mechanisms in insects, red blood cells, and lipid liposomes.
Journal of Biological Chemistry,
295 (28),
9606-9617.
Artículo
19 - Pacheco,S., Quiliche,J.P.J., Gomez,I., Sanchez,J., Soberon,M., Bravo,A. (2020). Rearrangement of N-Terminal alpha-Helices of Bacillus thuringiensis Cry1Ab Toxin Essential for Oligomer Assembly and Toxicity.
Toxins (Basel),
12 (10),
E647.
Artículo
20 - Gomez, I., Rodriguez-Chamorro, D.E., Flores-Ramirez, G., Grande, R., Zuniga, F., Portugal, F.J., Sanchez, J., Pacheco, S., Bravo, A., Soberon, M. (2018). Spodoptera frugiperda (J. E. Smith) aminopeptidase N1 is functional receptor of Bacillus thuringiensis Cry1Ca toxin.
Applied and Environmental Microbiology,
84 (17),
UNSP e01089-18.
Artículo
21 - Pacheco, S., Gomez, I., Sanchez, J., Garcia-Gomez, B.I., Czajkowsky, D.M., Zhang, J., Soberon, M., Bravo, A. (2018). Helix alpha-3 inter-molecular salt bridges and conformational changes are essential for toxicity of Bacillus thuringiensis 3D-Cry toxin family.
Scientific Reports,
8 (1),
10331.
Revisión
22 - Soberon, M., Portugal, L., Garcia-Gomez, B.I., Sanchez, J., Onofre, J., Gomez, I., Pacheco, S., Bravo, A. (2018). Cell lines as models for the study of Cry toxins from Bacillus thuringiensis.
Insect Biochemistry and Molecular Biology,
93,
66-78.
Artículo
23 - Torres-Quintero, M.C., Gomez, I., Pacheco, S., Sanchez, J., Flores, H., Osuna, J., Mendoza, G., Soberon, M., Bravo, A. (2018). Engineering Bacillus thuringiensis Cyt1Aa toxin specificity from dipteran to lepidopteran toxicity.
Scientific Reports,
8 (1),
4989.
Artículo
24 - Wang, Z., Fang, L., Zhou, Z., Pacheco, S., Gomez, I., Song, F., Soberon, M., Zhang, J., Bravo, A. (2018). Specific binding between Bacillus thuringiensis Cry9Aa and Vip3Aa toxins synergizes their toxicity against Asiatic rice borer (Chilo suppressalis).
Journal of Biological Chemistry,
293 (29),
11447-11458.
Artículo
25 - Ares, M.A., Rios-Sarabia, N., De la Cruz, M.A., Rivera-Gutierrez, S., Garcia-Morales, L., Leon-Solis, L., Espitia, C., Pacheco, S., Cerna-Cortes, J.F., Helguera-Repetto, C.A., Garcia, M.J., Gonzalez Y, Merchand J.A. (2017). The sigma factor SigD of Mycobacterium tuberculosis putatively enhances gene expression of the septum site determining protein under stressful environments.
New Microbiologica,
40 (3).
Artículo
26 - Ares, M.A., Fernandez-Vazquez, J.L., Pacheco, S., Martinez-Santos, V.I., Jarillo-Quijada, M.D., Torres, J., Alcantar-Curiel, M.D., Gonzalez Y Merchand J.A., De la Cruz, M.A. (2017). Additional regulatory activities of MrkH for the transcriptional expression of the Klebsiella pneumoniae mrk genes: Antagonist of H-NS and repressor.
PLoS ONE,
12 (3),
e0173285.
Artículo
27 - De la Cruz, M.A., Ruiz-Tagle, A., Ares, M.A., Pacheco, S., Yanez, J.A., Cedillo, L., Torres, J., Giron, J.A. (2017). The Expression of Longus type 4 pilus of enterotoxigenic Escherichia coli is regulated by LngR and LngS, and by H-NS, CpxR, and CRP global regulators.
Environmental Microbiology,
19 (5),
1761-1775.
Artículo; Actas Paper
28 - Onofre, J., Gaytan, M.O., Pena-Cardena, A., Garcia-Gomez, B.I., Pacheco, S., Gomez, I., Bravo, A., Soberon, M. (2017). Identification of Aminopeptidase-N2 as a Cry2Ab binding protein in Manduca sexta.
Peptides,
98,
93-98.
Artículo
29 - Pacheco, S., Gomez, I., Sanchez, J., Garcia-Gomez, B.I., Soberon, M., Bravo, A. (2017). Intra-molecular salt bridge in Bacillus thuringiensis Cry4Ba toxin is involved in the stability of helix alpha-3 that is needed for oligomerization and insecticidal activity.
Applied and Environmental Microbiology,
83 (20),
e01515-e01517.
Artículo
30 - Xiao, Y., Dai, Q., Hu, R., Pacheco, S., Yang, Y., Liang, G., Soberon, M., Bravo, A., Liu, K., Wu, K. (2017). A Single Point Mutation Resulting in Cadherin Mis-localization Underpins Resistance against Bacillus thuringiensis Toxin in Cotton Bollworm.
Journal of Biological Chemistry,
292 (7),
2933-2943.
Artículo
31 - Cardenas-Mondragon,M.G., Ares,M.A., Panunzi,L.G., Pacheco,S., Camorlinga-Ponce,M., Giron,J.A., Torres,J., De la Cruz,M.A. (2016). Transcriptional Profiling of Type II Toxin-Antitoxin Genes of Helicobacter pylori under Different Environmental Conditions: Identification of HP0967-HP0968 System.
Frontiers in Microbiology,
7,
1872.
Artículo
32 - Pacheco,S., Canton,E., Zuniga-Navarrete,F., Pecorari,F., Bravo,A., Soberon,M. (2015). Improvement and efficient display of Bacillus thuringiensis toxins on M13 phages and ribosomes.
AMB Express,
5 (1),
73.
Artículo
33 - Behar,G., Pacheco,S., Maillasson,M., Mouratou,B., Pecorari,F. (2014). Switching an anti-IgG binding site between archaeal extremophilic proteins results in Affitins with enhanced pH stability.
Journal of Biotechnology,
192 (Part A),
123-129.
*
Artículo
34 - Correa,A., Pacheco,S., Mechaly,A.E., Obal,G., Behar,G., Mouratou,B., Oppezzo,P., Alzari,P.M., Pecorari,F. (2014). Potent and specific inhibition of glycosidases by small artificial binding proteins (affitins).
PLoS ONE,
9 (5),
e97438.
*
Artículo
35 - Pacheco,S., Behar,G., Maillasson,M., Mouratou,B., Pecorari,F. (2014). Affinity transfer to the archaeal extremophilic Sac7d protein by insertion of a CDR.
Protein Engineering Design & Selection,
27 (10),
431-438.
*
Artículo
36 - Ares,M.A., Alcantar-Curiel,M.D., Jimenez-Galicia,C., Rios-Sarabia,N., Pacheco,S., De la Cruz,M.A. (2013). Antibiotic resistance of gram-negative bacilli isolated from pediatric patients with nosocomial bloodstream infections in a Mexican tertiary care hospital.
Chemotherapy,
59 (5),
361-368.
Artículo
37 - Cancino-Rodezno,A., Alexander,C., Villasenor,R., Pacheco,S., Porta,H., Pauchet,Y., Soberon,M., Gill,S.S., Bravo,A. (2010). The mitogen-activated protein kinase p38 is involved in insect defense against cry toxins from Bacillus thuringiensis.
Insect Biochemistry and Molecular Biology,
40 (1),
58-63 [corrigendum 43 (9) 898 2013 ].
Artículo
38 - Gomez,I., Arenas,I., Pacheco,S., Bravo,A., Soberon,M. (2010). New Insights into the Mode of Action of Cry1Ab Toxin Used in Transgenic Insect-resistant Crops.
Southwestern Entomologist,
35 (3),
387-390.
Artículo
39 - Pacheco,S., Gomez,I., Arenas,I., Saab-Rincon,G., Rodriguez-Almazan,C., Gill,S.S., Bravo,A., Soberon,M. (2009). Domain II loop 3 of Bacillus thuringiensis Cry1Ab toxin is involved in a "ping-pong" binding mechanism with Manduca sexta aminopetidase-N and cadherin receptors.
Journal of Biological Chemistry,
284 (47),
32750-32757.
Artículo
40 - Pacheco,S., Gomez,I., Gill,S.S., Bravo,A., Soberon,M. (2009). Enhancement of insecticidal activity of Bacillus thuringiensis Cry1A toxins by fragments of a toxin-binding cadherin correlates with oligomer formation.
Peptides,
30 (3),
583-588.
Artículo
41 - Rodriguez-Almazan,C., Zavala,L.E., Munoz-Garay,C., Jimenez-Juarez,N., Pacheco,S., Masson,L., Soberon,M., Bravo,A. (2009). Dominant negative mutants of Bacillus thuringiensis Cry1Ab toxin function as anti-toxins: demonstration of the role of oligomerization in toxicity.
PLoS ONE,
4 (5),
e5545 [Erratum 2013 8 (2)].
Artículo
42 - Fernandez,L.E., Gomez,I., Pacheco,S., Arenas,I., Gill,S.S., Bravo,A., Soberon,M. (2008). Employing phage display to study the mode of action of Bacillus thuringiensis Cry toxins.
Peptides,
29 (2),
324-329.
Artículo
43 - Pacheco,S., Gomez,I., Sato,R., Bravo,A., Soberon,M. (2006). Functional display of Bacillus thuringiensis Cry1Ac toxin on T7 phage.
Journal of Invertebrate Pathology,
92 (1),
45-49.
Internacional
44 - Bravo,A., Pacheco,S., Gomez,I., Soberon,M. (2023). Mode of action of Bacillus thuringiensis Cry pesticidal proteins. Insecticidal Proteins and RNAi in the Control of Insects.
Advances in Insect Physiology,
55-92,
65,
Cambridge, Mass.: Academic Press Inc..
Internacional
45 - Bravo, A., Pacheco, S., Gomez, I., Garcia-Gomez, B., Onofre, J., Soberon, M. (2017). Insecticidal Proteins from Bacillus thuringiensis and Their Mechanism of Action. Bacillus thuringiensis and Lysinibacillus sphaericus: Characterization and use in the field of biocontrol.
53-66,
Cham: Springer.
Internacional
46 - Bravo, A., Martinez-de-Castro, D. L., Sanchez-Quintana, J., Canton, P. E., Mendoza, G., Gomez, I., Pacheco, S., Garcia-Gomez, B. I., Onofre, J., Ocelotl, J., Soberon, M. (2015). Mechanism of action of Bacillus thuringiensis insecticidal toxins and their use in the control of insect pests. Comprehensive Sourcebook of Bacterial Protein Toxins.
858-873,
4a ed.
Internacional
47 - Soberon,M., Garcia-Gomez,B.I., Pacheco,S., Sanchez-Quintana,J., Tabashnik,B.E., Bravo,A. (2015). Countering pest resistance with genetically modified Bt toxins. Bt resistance-characterization and strategies for GM crops expressing Bacillus thuringiensis.
CABI Biotechnology Series Volume: 4,
150-161,
Oxford.
Internacional
48 - Pacheco,S., Soberon,M. (2012). Phage display: Fundamentals and applications. Tools to Understand Protein-Protein Interactions.
143,
Kerala: Research Signpost.
Nacional divulgación
49 - Pelaez-Aguilar,A.E., Pacheco,S., Gomez,I. (2025). La biotecnología acechando a los mosquitos.
Biotecnología en Movimiento.Revista de divulgación del Instituto de Biotecnología de la UNAM,
43.
*
nacional divulgacion
50 - Gomez,I., Pacheco,S. (2022). La bacteria Bacillus thuringiensis como bioinsecticida en la agricultura actual.
Biotecnología en Movimiento. Revista de divulgación del Instituto de Biotecnología de la UNAM,
29.
51 - Pacheco Guillen, Sabino (2010). Papel del asa 3 del dominio II de las toxinas Cry1A's de Bacillus thuringiensis en el mecanismo de toxicidad : un blanco potencial para modificar el reconocimiento de sus receptores.
Instituto de Biotecnologia,
Doctorado en Ciencias Bioquimicas,
UNAM.
Asesor: Soberon Chavez, Mario
Artículo
52 - do Nascimento,N.A., Torres-Quintero,M.C., Garcia-Gomez,B.I., Gomez,I., Pacheco,S., Bravo,A., Soberon,M.. Engineering Bacillus thuringiensis Cyt1Aa to function as a surrogate receptor of Cry1A lepidopteran insecticidal proteins.
Pesticide Biochemistry And Physiology,
219 (April),
106970,
[Correction 107201 2026].
Artículo
53 - Gomez,I., Garcia-Gomez,B.I., do Nascimento,N.A., Infante,O., Canton,P.E., Pacheco,S., Pelaez-Aguilar,A.E., Sanchez,J., Soberon,M., Bravo,A.. Regulatory diversity in Bacillus thuringiensis cry genes reveals flexible evolutionary strategies for in vivo toxin expression.
PLoS Pathogens,
22 (3),
e1014017.
Artículo
54 - Quintana,A.J., Garcia-Suarez,R., Prieto,A., Sanchez,J., Gomez,I., Verduzco-Rosas,L.A., doNascimento,N., Lopez-Molina,S., Zhang,J., Soberon,M., Bravo,A., Pacheco,S.. Genome editing of ABCB6 Transporter Confers Resistance to Cypermethrin in the Major Pest of Corn, Spodoptera frugiperda.
Insect Biochemistry and Molecular Biology,
190 (May),
104537.
Artículo
55 - Lopez-Molina,S., Guerrero,A., Pacheco,S., Wang,Z., Zhang,J., Sanchez,J., Zavala,G., Soberon,M., Bravo,A.. Cry11Aa toxin of Bacillus thuringiensis interactions with intracellular organelles in insect gut implicating actin depolymerization, massive endocytosis, and vesicle secretion.
International Journal of Biological Macromolecules,
314,
144350.
Artículo
56 - Pacheco,S., Chinas,M., Gomez,J.U., Pelaez-Aguilar,A.E., do Nascimento,N.A., Canton,P.E., Sanchez,J., Lopez-Molina,S., Gomez,I., Soberon,M., Bravo,A.. ABC transporters knockout in Aedes aegypti induces upregulation of paralogous genes, avoiding resistance development to Bacillus thuringiensis Cry toxins.
PLoS ONE,
20 (7),
e0327221.
Nacional divulgación
57 - Pelaez-Aguilar,A.E., Pacheco,S., Gomez,I.. La biotecnología acechando a los mosquitos.
Biotecnología en Movimiento.Revista de divulgación del Instituto de Biotecnología de la UNAM,
43.
*
Artículo
58 - Pacheco,S., Gallegos,A.S., Pelaez-Aguilar,A.E., Sanchez,J., Gomez,I., Soberon,M., Bravo,A.. CRISPR-Cas9 knockout of membrane-bound alkaline phosphatase or cadherin does not confer resistance to Cry toxins in Aedes aegypti.
PLoS Neglected Tropical Diseases,
18 (6),
e0012256.
Revisión
59 - Pacheco,S., Gomez,I., Pelaez-Aguilar,A.E., Verduzco-Rosas,L.A., Garcia-Suarez,R., do Nascimento,N.A., Rivera-Najera,L.Y., Canton,P.E., Soberon,M., Bravo,A.. Structural changes upon membrane insertion of the insecticidal pore-forming toxins produced by Bacillus thuringiensis.
Frontiers in Insect Science,
3,
1188891.
Artículo
60 - Pacheco,S., Gomez,I., Soberon,M., Bravo,A.. A major conformational change of N-terminal helices of Bacillus thuringiensis Cry1Ab insecticidal protein is necessary for membrane insertion and toxicity.
Febs Journal,
290 (10),
2692-2705.
Internacional
61 - Bravo,A., Pacheco,S., Gomez,I., Soberon,M.. Mode of action of Bacillus thuringiensis Cry pesticidal proteins. Insecticidal Proteins and RNAi in the Control of Insects.
Advances in Insect Physiology,
55-92,
65,
Cambridge, Mass.: Academic Press Inc..
nacional divulgacion
62 - Gomez,I., Pacheco,S.. La bacteria Bacillus thuringiensis como bioinsecticida en la agricultura actual.
Biotecnología en Movimiento. Revista de divulgación del Instituto de Biotecnología de la UNAM,
29.
Artículo
63 - Lopez-Molina,S., do Nascimento,N.A., Silva-Filha,M.H.N.L., Guerrero,A., Sanchez,J., Pacheco,S., Gill,S.S., Soberon,M., Bravo,A.. In vivo nanoscale analysis of the dynamic synergistic interaction of Bacillus thuringiensis Cry11Aa and Cyt1Aa toxins in Aedes aegypti.
PLoS Pathogens,
17 (1),
e1009199.
Artículo
64 - Pacheco,S., Gomez,I., Chinas,M., Sanchez,J., Soberon,M., Bravo,A.. Whole Genome Sequencing Analysis of Bacillus thuringiensis GR007 Reveals Multiple Pesticidal Protein Genes.
Frontiers in Microbiology,
12,
3268.
Artículo
65 - Sena da Silva,I.H., Gomez,I., Pacheco,S., Sanchez,J., Zhang,J., Luque Castellane,T.C., Aparecida Desiderio J., Soberon,M., Bravo,A., Polanczyk,R.A.. Bacillus thuringiensis Cry1Ab domain III -16 is involved in binding to prohibitin which correlates with toxicity against Helicoverpa armigera (Lepidoptera: Noctuidae).
Applied and Environmental Microbiology,
87 (2),
e01930-20.
Artículo
66 - Chimal-Cazares,F., Hernandez-Martinez,G., Pacheco,S., Ares,M.A., Soria-Bustos,J., Sanchez-Gutierrez,M., Izquierdo-Vega,J.A., Ibarra,J.A., Gonzalez,Y.Merchand JA, Gorvel,J.P., Meresse,S., De la Cruz,M.A.. Molecular Characterization of SehB, a Type II Antitoxin of Salmonella enterica Serotype Typhimurium: Amino Acid Residues Involved in DNA-Binding, Homodimerization, Toxin Interaction, and Virulence.
Frontiers in Microbiology,
11,
614.
Artículo
67 - do Nascimento,N.A., Torres-Quintero,M.C., Lopez-Molina,S., Pacheco,S., Romao,T.P., Pereira-Neves,A., Soberon,M., Bravo,A., Silva-Filha,M.H.N.L.. Functional Bacillus thuringiensis Cyt1Aa is necessary to synergize Lysinibacillus sphaericus Binary toxin against Bin-resistant and refractory mosquito species.
Applied and Environmental Microbiology,
86 (7),
02770-19.
Artículo
68 - Onofre,J., Pacheco,S., Torres-Quintero,M.C., Gill,S.S., Soberon,M., Bravo,A.. The Cyt1Aa toxin from Bacillus thuringiensis inserts into target membranes via different mechanisms in insects, red blood cells, and lipid liposomes.
Journal of Biological Chemistry,
295 (28),
9606-9617.
Artículo
69 - Pacheco,S., Quiliche,J.P.J., Gomez,I., Sanchez,J., Soberon,M., Bravo,A.. Rearrangement of N-Terminal alpha-Helices of Bacillus thuringiensis Cry1Ab Toxin Essential for Oligomer Assembly and Toxicity.
Toxins (Basel),
12 (10),
E647.
Artículo
70 - Gomez, I., Rodriguez-Chamorro, D.E., Flores-Ramirez, G., Grande, R., Zuniga, F., Portugal, F.J., Sanchez, J., Pacheco, S., Bravo, A., Soberon, M.. Spodoptera frugiperda (J. E. Smith) aminopeptidase N1 is functional receptor of Bacillus thuringiensis Cry1Ca toxin.
Applied and Environmental Microbiology,
84 (17),
UNSP e01089-18.
Artículo
71 - Pacheco, S., Gomez, I., Sanchez, J., Garcia-Gomez, B.I., Czajkowsky, D.M., Zhang, J., Soberon, M., Bravo, A.. Helix alpha-3 inter-molecular salt bridges and conformational changes are essential for toxicity of Bacillus thuringiensis 3D-Cry toxin family.
Scientific Reports,
8 (1),
10331.
Revisión
72 - Soberon, M., Portugal, L., Garcia-Gomez, B.I., Sanchez, J., Onofre, J., Gomez, I., Pacheco, S., Bravo, A.. Cell lines as models for the study of Cry toxins from Bacillus thuringiensis.
Insect Biochemistry and Molecular Biology,
93,
66-78.
Artículo
73 - Torres-Quintero, M.C., Gomez, I., Pacheco, S., Sanchez, J., Flores, H., Osuna, J., Mendoza, G., Soberon, M., Bravo, A.. Engineering Bacillus thuringiensis Cyt1Aa toxin specificity from dipteran to lepidopteran toxicity.
Scientific Reports,
8 (1),
4989.
Artículo
74 - Wang, Z., Fang, L., Zhou, Z., Pacheco, S., Gomez, I., Song, F., Soberon, M., Zhang, J., Bravo, A.. Specific binding between Bacillus thuringiensis Cry9Aa and Vip3Aa toxins synergizes their toxicity against Asiatic rice borer (Chilo suppressalis).
Journal of Biological Chemistry,
293 (29),
11447-11458.
Artículo
75 - Ares, M.A., Rios-Sarabia, N., De la Cruz, M.A., Rivera-Gutierrez, S., Garcia-Morales, L., Leon-Solis, L., Espitia, C., Pacheco, S., Cerna-Cortes, J.F., Helguera-Repetto, C.A., Garcia, M.J., Gonzalez Y, Merchand J.A.. The sigma factor SigD of Mycobacterium tuberculosis putatively enhances gene expression of the septum site determining protein under stressful environments.
New Microbiologica,
40 (3).
Artículo
76 - Ares, M.A., Fernandez-Vazquez, J.L., Pacheco, S., Martinez-Santos, V.I., Jarillo-Quijada, M.D., Torres, J., Alcantar-Curiel, M.D., Gonzalez Y Merchand J.A., De la Cruz, M.A.. Additional regulatory activities of MrkH for the transcriptional expression of the Klebsiella pneumoniae mrk genes: Antagonist of H-NS and repressor.
PLoS ONE,
12 (3),
e0173285.
Artículo
77 - De la Cruz, M.A., Ruiz-Tagle, A., Ares, M.A., Pacheco, S., Yanez, J.A., Cedillo, L., Torres, J., Giron, J.A.. The Expression of Longus type 4 pilus of enterotoxigenic Escherichia coli is regulated by LngR and LngS, and by H-NS, CpxR, and CRP global regulators.
Environmental Microbiology,
19 (5),
1761-1775.
Artículo; Actas Paper
78 - Onofre, J., Gaytan, M.O., Pena-Cardena, A., Garcia-Gomez, B.I., Pacheco, S., Gomez, I., Bravo, A., Soberon, M.. Identification of Aminopeptidase-N2 as a Cry2Ab binding protein in Manduca sexta.
Peptides,
98,
93-98.
Artículo
79 - Pacheco, S., Gomez, I., Sanchez, J., Garcia-Gomez, B.I., Soberon, M., Bravo, A.. Intra-molecular salt bridge in Bacillus thuringiensis Cry4Ba toxin is involved in the stability of helix alpha-3 that is needed for oligomerization and insecticidal activity.
Applied and Environmental Microbiology,
83 (20),
e01515-e01517.
Artículo
80 - Xiao, Y., Dai, Q., Hu, R., Pacheco, S., Yang, Y., Liang, G., Soberon, M., Bravo, A., Liu, K., Wu, K.. A Single Point Mutation Resulting in Cadherin Mis-localization Underpins Resistance against Bacillus thuringiensis Toxin in Cotton Bollworm.
Journal of Biological Chemistry,
292 (7),
2933-2943.
Internacional
81 - Bravo, A., Pacheco, S., Gomez, I., Garcia-Gomez, B., Onofre, J., Soberon, M.. Insecticidal Proteins from Bacillus thuringiensis and Their Mechanism of Action. Bacillus thuringiensis and Lysinibacillus sphaericus: Characterization and use in the field of biocontrol.
53-66,
Cham: Springer.
Artículo
82 - Cardenas-Mondragon,M.G., Ares,M.A., Panunzi,L.G., Pacheco,S., Camorlinga-Ponce,M., Giron,J.A., Torres,J., De la Cruz,M.A.. Transcriptional Profiling of Type II Toxin-Antitoxin Genes of Helicobacter pylori under Different Environmental Conditions: Identification of HP0967-HP0968 System.
Frontiers in Microbiology,
7,
1872.
Artículo
83 - Pacheco,S., Canton,E., Zuniga-Navarrete,F., Pecorari,F., Bravo,A., Soberon,M.. Improvement and efficient display of Bacillus thuringiensis toxins on M13 phages and ribosomes.
AMB Express,
5 (1),
73.
Internacional
84 - Bravo, A., Martinez-de-Castro, D. L., Sanchez-Quintana, J., Canton, P. E., Mendoza, G., Gomez, I., Pacheco, S., Garcia-Gomez, B. I., Onofre, J., Ocelotl, J., Soberon, M.. Mechanism of action of Bacillus thuringiensis insecticidal toxins and their use in the control of insect pests. Comprehensive Sourcebook of Bacterial Protein Toxins.
858-873,
4a ed.
Internacional
85 - Soberon,M., Garcia-Gomez,B.I., Pacheco,S., Sanchez-Quintana,J., Tabashnik,B.E., Bravo,A.. Countering pest resistance with genetically modified Bt toxins. Bt resistance-characterization and strategies for GM crops expressing Bacillus thuringiensis.
CABI Biotechnology Series Volume: 4,
150-161,
Oxford.
Artículo
86 - Behar,G., Pacheco,S., Maillasson,M., Mouratou,B., Pecorari,F.. Switching an anti-IgG binding site between archaeal extremophilic proteins results in Affitins with enhanced pH stability.
Journal of Biotechnology,
192 (Part A),
123-129.
*
Artículo
87 - Correa,A., Pacheco,S., Mechaly,A.E., Obal,G., Behar,G., Mouratou,B., Oppezzo,P., Alzari,P.M., Pecorari,F.. Potent and specific inhibition of glycosidases by small artificial binding proteins (affitins).
PLoS ONE,
9 (5),
e97438.
*
Artículo
88 - Pacheco,S., Behar,G., Maillasson,M., Mouratou,B., Pecorari,F.. Affinity transfer to the archaeal extremophilic Sac7d protein by insertion of a CDR.
Protein Engineering Design & Selection,
27 (10),
431-438.
*
Artículo
89 - Ares,M.A., Alcantar-Curiel,M.D., Jimenez-Galicia,C., Rios-Sarabia,N., Pacheco,S., De la Cruz,M.A.. Antibiotic resistance of gram-negative bacilli isolated from pediatric patients with nosocomial bloodstream infections in a Mexican tertiary care hospital.
Chemotherapy,
59 (5),
361-368.
Internacional
90 - Pacheco,S., Soberon,M.. Phage display: Fundamentals and applications. Tools to Understand Protein-Protein Interactions.
143,
Kerala: Research Signpost.
Artículo
91 - Cancino-Rodezno,A., Alexander,C., Villasenor,R., Pacheco,S., Porta,H., Pauchet,Y., Soberon,M., Gill,S.S., Bravo,A.. The mitogen-activated protein kinase p38 is involved in insect defense against cry toxins from Bacillus thuringiensis.
Insect Biochemistry and Molecular Biology,
40 (1),
58-63 [corrigendum 43 (9) 898 2013 ].
Artículo
92 - Gomez,I., Arenas,I., Pacheco,S., Bravo,A., Soberon,M.. New Insights into the Mode of Action of Cry1Ab Toxin Used in Transgenic Insect-resistant Crops.
Southwestern Entomologist,
35 (3),
387-390.
93 - Pacheco Guillen, Sabino. Papel del asa 3 del dominio II de las toxinas Cry1A's de Bacillus thuringiensis en el mecanismo de toxicidad : un blanco potencial para modificar el reconocimiento de sus receptores.
Instituto de Biotecnologia,
Doctorado en Ciencias Bioquimicas,
UNAM.
Asesor: Soberon Chavez, Mario
Artículo
94 - Pacheco,S., Gomez,I., Arenas,I., Saab-Rincon,G., Rodriguez-Almazan,C., Gill,S.S., Bravo,A., Soberon,M.. Domain II loop 3 of Bacillus thuringiensis Cry1Ab toxin is involved in a "ping-pong" binding mechanism with Manduca sexta aminopetidase-N and cadherin receptors.
Journal of Biological Chemistry,
284 (47),
32750-32757.
Artículo
95 - Pacheco,S., Gomez,I., Gill,S.S., Bravo,A., Soberon,M.. Enhancement of insecticidal activity of Bacillus thuringiensis Cry1A toxins by fragments of a toxin-binding cadherin correlates with oligomer formation.
Peptides,
30 (3),
583-588.
Artículo
96 - Rodriguez-Almazan,C., Zavala,L.E., Munoz-Garay,C., Jimenez-Juarez,N., Pacheco,S., Masson,L., Soberon,M., Bravo,A.. Dominant negative mutants of Bacillus thuringiensis Cry1Ab toxin function as anti-toxins: demonstration of the role of oligomerization in toxicity.
PLoS ONE,
4 (5),
e5545 [Erratum 2013 8 (2)].
Artículo
97 - Fernandez,L.E., Gomez,I., Pacheco,S., Arenas,I., Gill,S.S., Bravo,A., Soberon,M.. Employing phage display to study the mode of action of Bacillus thuringiensis Cry toxins.
Peptides,
29 (2),
324-329.
Artículo
98 - Pacheco,S., Gomez,I., Sato,R., Bravo,A., Soberon,M.. Functional display of Bacillus thuringiensis Cry1Ac toxin on T7 phage.
Journal of Invertebrate Pathology,
92 (1),
45-49.