Dra. Noemi Flores Mejia
Técnica Académica Titular C Tiempo Completo
Ingeniería y Tecnología de las Fermentaciones y del Cultivo Celular
Nivel D del PRIDE
- Graduados
1 - Cruz Tinoco, Fernando (2024). Manual de las técnicas de purificación de RNA y PCR en tiempo real para bacterias.
Ingeniería en Biotecnología,
Universidad Politécnica del Estado de Morelos.
*
Asesor: Flores Mejia,Noemi
2 - Pillado Cruz, María del Pilar (2024). Técnicas básicas de Biología Molecular en Eschericha coli.
Ingeniería en Biotecnología,
Universidad Politécnica del Estado de Morelos.
*
Asesor: Flores Mejia, Noemi
Total de publicaciones: 52
- Tipo
- Fecha
Artículo in press communication
3 - Pohlen,L., Garcia,E., Martinez,L.M., FLORES,N., Buchs,J., Gosset,G., Lara,A.R. (2025). Sigma factors as potential targets to enhance recombinant protein expression.
Biotechnology and Bioengineering,
Feb 24 [Online ahead of print].
Artículo in press
4 - Vallejo-Giraldo,A., Martinez,L.M., FLORES,N., Hernandez-Chavez,G., Cueto,F., Martinez,A., Corona-Gonzalez,R.I., Gosset,G. (2025). Construction and Characterization of Novel Shuttle Expression Vectors for Actinobacillus succinogenes.
Biotechnology and Applied Biochemistry,
Feb 17 [Online ahead of print].
Artículo
5 - Escalante,A., Astudillo,F., Larios,V., FLORES,N., Gosset,G., Bolivar,F. (2024). The complete genome of Escherichia coli JM101 assembled with a combination of Nanopore and Illumina platforms.
Microbiology Resource Announcements,
13 (2),
e0097323.
Artículo
Artículo
7 - Fragoso-Jimenez,J.C., Gutierrez-Rios,R.M., FLORES,N., Martinez,A., Lara,A.R., Delvigne,F., Gosset,G. (2022). Glucose consumption rate-dependent transcriptome profiling of Escherichia coli provides insight on performance as microbial factories.
Microbial Cell Factories,
21 (1),
189.
Artículo
8 - Rodriguez-Ochoa,J.I., Fragoso-Jimenez,J.C., FLORES,N., Martinez,L.M., Bolivar,F., Martinez,A., Gosset,G. (2022). Global transcriptomic response of Escherichia coli to p-coumaric acid.
Microbial Cell Factories,
21 (1),
148.
Artículo
9 - Carmona,S.B., FLORES,N., Martinez-Romero,E., Gosset,G., Bolivar,F., Escalante,A. (2020). Evolution of an Escherichia coli PTS(-) strain: a study of reproducibility and dynamics of an adaptive evolutive process.
Applied Microbiology and Biotechnology,
104,
9309?9325.
Artículo
10 - Cristiano-Fajardo,S.A., Flores,C., FLORES,N., Tinoco-Valencia,R., Serrano-Carreon,L., Galindo,E. (2019). Glucose limitation and glucose uptake rate determines metabolite production and sporulation in high cell density continuous cultures of Bacillus amyloliquefaciens 83.
Journal of Biotechnology,
299,
57-65.
Artículo
11 - Aguilar,C., Martinez-Batallar,G., FLORES,N., Moreno-Avitia,F., Encarnacion,S., Escalante,A., Bolivar,F. (2018). Analysis of differentially upregulated proteins in ptsHIcrr(-) and rppH(-) mutants in Escherichia coli during an adaptive laboratory evolution experiment.
Applied Microbiology and Biotechnology,
102 (23),
10193?10208 .
Artículo
12 - Martinez,J.A., Rodriguez,A., Moreno,F., FLORES,N., Lara,A.R., Ramirez,O.T., Gosset,G., Bolivar,F. (2018). Metabolic modeling and response surface analysis of an Escherichia coli strain engineered for shikimic acid production.
BMC Systems Biology,
12 (1),
102.
Artículo
13 - Salas-Navarrete, C., Hernandez-Chavez, G., Flores, N., Martinez, L.M., Martinez, A., Bolivar, F., Barona-Gomez, F., Gosset, G. (2018). Increasing pinosylvin production in Escherichia coli by reducing the expression level of the gene fabI-encoded enoyl-acyl carrier protein reductase.
Electronic Journal of Biotechnology,
33,
11-16.
Artículo
14 - Garcia, S., Flores, N., de Anda, R., Hernandez, G., Gosset, G., Bolivar, F., Escalante, A. (2017). The Role of the ydiB Gene, Which Encodes Quinate/Shikimate Dehydrogenase, in the Production of Quinic, Dehydroshikimic and Shikimic Acids in a PTS- Strain of Escherichia coli.
Journal of Molecular Microbiology and Biotechnology,
27 (1),
11-21.
Artículo
15 - Cortes,J.T., FLORES,N., Bolivar,F., Lara,A.R., Ramirez,O.T. (2016). Physiological effects of pH gradients on Escherichia coli during plasmid DNA production.
Biotechnology and Bioengineering,
113 (3),
598-611.
Artículo
16 - Fernandez-Alejandre,K.I., FLORES,N., Tinoco-Valencia,R., Caro,M., Flores,C., Galindo,E., Serrano-Carreon,L. (2016). Diffusional and transcriptional mechanisms involved in laccases production by Pleurotus ostreatus CP50.
Journal of Biotechnology,
223,
42-49.
Artículo
17 - Aguilar,C., FLORES,N., Riveros-McKay,F., Sahonero-Canavesi,D., Carmona,S.B., Geiger,O., Escalante,A., Bolivar,F. (2015). Deletion of the 2-acyl-glycerophosphoethanolamine cycle improve glucose metabolism in Escherichia coli strains employed for overproduction of aromatic compounds.
Microbial Cell Factories,
14 (1),
194.
Revisión
18 - Rodriguez,A., Martinez,J.A., FLORES,N., Escalante,A., Gosset,G., Bolivar,F. (2014). Engineering Escherichia coli to overproduce aromatic amino acids and derived compounds.
Microbial Cell Factories,
13 (1),
126.
Artículo
19 - Sabido,A., Sigala,J.C., Hernandez-Chavez,G., FLORES,N., Gosset,G., Bolivar,F. (2014). Physiological and transcriptional characterization of Escherichia coli strains lacking interconversion of phosphoenolpyruvate and pyruvate when glucose and acetate are coutilized.
Biotechnology and Bioengineering,
111 (6),
1150-1160.
Artículo
20 - Caspeta,L., Lara,A.R., Perez,N.O., FLORES,N., Bolivar,F., Ramirez,O.T. (2013). Enhancing thermo-induced recombinant protein production in Escherichia coli by temperature oscillations and post-induction nutrient feeding strategies.
Journal of Biotechnology,
167 (1),
47-55.
Artículo
21 - Centeno-Leija,S., Utrilla,J., FLORES,N., Rodriguez,A., Gosset,G., Martinez,A. (2013). Metabolic and transcriptional response of Escherichia coli with a NADP-dependent glyceraldehyde 3-phosphate dehydrogenase from Streptococcus mutans.
Antonie Van Leeuwenhoek,
104 (6),
913-924.
Artículo
22 - Rodriguez,A., Martinez,J.A., Baez-Viveros,J.L., FLORES,N., Hernandez-Chavez,G., Ramirez,O.T., Gosset,G., Bolivar,F. (2013). Constitutive expression of selected genes from the pentose phosphate and aromatic pathways increases the shikimic acid yield in high-glucose batch cultures of an Escherichia coli strain lacking PTS and pykF.
Microbial Cell Factories,
12 (1),
86.
Artículo
23 - Soria,S., de Anda,R., FLORES,N., Romero-Garcia,S., Gosset,G., Bolivar,F., Baez-Viveros,J.L. (2013). New insights on transcriptional responses of genes involved in carbon central metabolism, respiration and fermentation to low ATP levels in Escherichia coli.
Journal of Basic Microbiology,
53 (4),
365-380.
Artículo
24 - Aguilar,C., Escalante,A., FLORES,N., de Anda,R., Riveros-McKay,F., Gosset,G., Morett,E., Bolivar,F. (2012). Genetic changes during a laboratory adaptive evolution process that allowed fast growth in glucose to an Escherichia coli strain lacking the major glucose transport system.
BMC Genomics,
13 (1),
385.
Artículo
25 - Cabrera-Valladares,N., Martinez,L.M., FLORES,N., Hernandez-Chavez,G., Martinez,A., Bolivar,F., Gosset,G. (2012). Physiologic Consequences of Glucose Transport and Phosphoenolpyruvate Node Modifications in Bacillus subtilis 168.
Journal of Molecular Microbiology and Biotechnology,
22 (3),
177-197.
Artículo
26 - Martinez-Gomez,K., FLORES,N., Castaneda,H.M., Martinez-Batallar,G., Hernandez-Chavez,G., Ramirez,O.T., Gosset,G., Encarnacion,S., Bolivar,F. (2012). New insights into Escherichia coli metabolism: carbon scavenging, acetate metabolism and carbon recycling responses during growth on glycerol.
Microbial Cell Factories,
11 (1),
46.
Artículo
27 - Baez,A., FLORES,N., Bolivar,F., Ramirez,O.T. (2011). Simulation of dissolved CO(2) gradients in a scale-down system: A metabolic and transcriptional study of recombinant Escherichia coli.
Biotechnology Journal,
6 (8),
959-967.
Artículo
28 - Baez,A., FLORES,N., Bolivar,F., Ramirez,O.T. (2009). Metabolic and transcriptional response of recombinant Escherichia coli to elevated dissolved carbon dioxide concentrations.
Biotechnology and Bioengineering,
104 (1),
102-110.
Artículo
29 - Caspeta,L., FLORES,N., Perez,N.O., Bolivar,F., Ramirez,O.T. (2009). The effect of heating rate on Escherichia coli metabolism, physiological stress, transcriptional response, and production of temperature-induced recombinant protein: a scale-down study.
Biotechnology and Bioengineering,
102 (2),
468-482.
Artículo
Artículo
31 - Sigala,J.C., Flores,S., FLORES,N., Aguilar,C., de Anda,R., Gosset,G., Bolivar,F. (2009). Acetate Metabolism in Escherichia coli Strains Lacking Phosphoenolpyruvate: Carbohydrate Phosphotransferase System; Evidence of Carbon Recycling Strategies and Futile Cycles.
Journal of Molecular Microbiology and Biotechnology,
16 (3-4),
224-235.
Artículo
32 - FLORES,N., Escalante,A., de Anda,R., Baez-Viveros,J.L., Merino,E., Franco,B., Georgellis,D., Gosset,G., Bolivar,F. (2008). New Insights into the Role of Sigma Factor RpoS as Revealed in Escherichia coli Strains Lacking the Phosphoenolpyruvate:Carbohydrate Phosphotransferase System.
Journal of Molecular Microbiology and Biotechnology,
14 (4),
176-192.
Artículo
33 - Orencio-Trejo,M., FLORES,N., Escalante,A., Hernandez-Chavez,G., Bolivar,F., Gosset,G., Martinez,A. (2008). Metabolic regulation analysis of an ethanologenic Escherichia coli strain based on RT-PCR and enzymatic activities.
Biotechnology for Biofuels,
1 (1),
8.
Artículo
34 - Baez-Viveros,J.L., FLORES,N., Suarez,K., Castillo-Espana,P., Bolivar,F., Gosset,G. (2007). Metabolic transcription analysis of engineered Escherichia coli strains that overproduce L-phenylalanine.
Microbial Cell Factories,
6 (1),
30.
Artículo
35 - FLORES,N., Leal,L., Sigala,J.C., de Anda,R., Escalante,A., Martinez,A., Ramirez,O.T., Gosset,G., Bolivar,F. (2007). Growth Recovery on Glucose under Aerobic Conditions of an Escherichia coli Strain Carrying a Phosphoenolpyruvate:Carbohydrate Phosphotransferase System Deletion by Inactivating arcA and Overexpressing the Genes Coding for Glucokinase and Galactose Permease.
Journal of Molecular Microbiology and Biotechnology,
13 (1-3),
105-116.
Artículo
36 - Lara,A.R., Leal,L., FLORES,N., Gosset,G., Bolivar,F., Ramirez,O.T. (2006). Transcriptional and metabolic response of recombinant Escherichia coli to spatial dissolved oxygen tension gradients simulated in a scale-down system.
Biotechnology and Bioengineering,
93 (2),
372-385.
Artículo
37 - FLORES,N., Flores,S., Escalante,A., de Anda,R., Leal,L., Malpica,R., Georgellis,D., Gosset,G., Bolivar,F. (2005). Adaptation for fast growth on glucose by differential expression of central carbon metabolism and gal regulon genes in an Escherichia coli strain lacking the phosphoenolpyruvate:carbohydrate phosphotransferase system.
Metabolic Engineering,
7 (2),
70-87.
Artículo
38 - Flores,S., FLORES,N., de Anda,R., Gonzalez,A., Escalante,A., Sigala,J.C., Gosset,G., Bolivar,F. (2005). Nutrient-Scavenging Stress Response in an Escherichia coli Strain Lacking the Phosphoenolpyruvate:Carbohydrate Phosphotransferase System, as Explored by Gene Expression Profile Analysis.
Journal of Molecular Microbiology and Biotechnology,
10 (1),
51-63.
Artículo
39 - FLORES,N., de Anda,R., Flores,S., Escalante,A., Hernandez,G., Martinez,A., Ramirez,O.T., Gosset,G., Bolivar,F. (2004). Role of Pyruvate Oxidase in Escherichia coli Strains Lacking the Phosphoenolpyruvate:Carbohydrate Phosphotransferase System.
Journal of Molecular Microbiology and Biotechnology,
8 (4),
209-221.
Artículo
40 - Flores,S., Gosset,G., FLORES,N., de Graaf,A.A., Bolivar,F. (2002). Analysis of carbon metabolism in Escherichia coli strains with an inactive phosphotransferase system by 13C labelling and NMR spectroscopy.
Metabolic Engineering,
4 (2),
124-137.
Artículo
41 - Siguenza,R., FLORES,N., Hernandez,G., Martinez,A., Bolivar,F., Valle,F. (1999). Kinetic characterization in batch and continuous culture of Escherichia coli mutants affected in phosphoenolpyruvate metabolism: differences in acetic acid production.
World Journal of Microbiology & Biotechnology,
15 (5),
587-592.
Artículo
42 - FLORES,N., Xiao,J., Berry,A., Bolivar,F., Valle,F. (1996). Pathway engineering for the production of aromatic compounds in Escherichia coli.
Nature Biotechnology,
14 (5),
620-623.
Artículo
43 - Valle,F., Munoz,E., Ponce,E., FLORES,N., Bolivar,F. (1996). Basic and applied aspects of metabolic diversity: The phosphoenolpyruvate node.
Journal of Industrial Microbiology & Biotechnology,
17 (5-6),
458-462.
Note
44 - Ponce,E., FLORES,N., Martinez,A., Valle,F., Bolivar,F. (1995). Cloning of the two pyruvate kinase isoenzyme structural genes from Escherichia coli: the relative roles of these enzymes in pyruvate biosynthesis.
Journal of Bacteriology,
177 (19),
5719-5722.
Artículo
45 - Castano,I., FLORES,N., Valle,F., Covarrubias,A.A., Bolivar,F. (1992). gltF, a member of the gltBDF operon of Escherichia coli, is involved in nitrogen-regulated gene expression.
Molecular Microbiology,
6 (18),
2733-2741.
Artículo
46 - FLORES,N., Valle,F., Bolivar,F., Merino,E. (1992). Recovery of DNA from agarose gels stained with methylene blue.
Biotechniques,
13 (2),
203-205.
Artículo
47 - Oliver,G., Gosset,G., Sanchez-Pescador,R., Lozoya,E., Ku,L.M., FLORES,N., Becerril,B., Valle,F., Bolivar,F. (1987). Determination of the nucleotide sequence for the glutamate synthase structural genes of Escherichia coli K-12.
Gene,
60 (1),
1-11.
Artículo
48 - Balbas,P., Soberon,X., Merino,E., Zurita,M., Lomeli,H., Valle,F., FLORES,N., Bolivar,F. (1986). Plasmid vector pBR322 and its special-purpose derivatives--a review.
Gene,
50 (1-3),
3-40.
Artículo
49 - FLORES,N., de Anda,R., Guereca,L., Cruz,N., Antonio,S., Balbas,P., Bolivar,F., Valle,F. (1986). A new expression vector for the production of fused proteins in Escherichia coli.
Applied Microbiology and Biotechnology,
25 (3),
267-271.
Memoria in extenso
50 - Balbas,P., de Anda,R., FLORES,N., Alvarado,X., Cruz,N., Valle,F., Bolivar,F. (1988). Overproduction of proteins by recombinant DNA: human insulin. Cell Function and Disease.
59-74,
New York.
Preprint
51 - Pohlen,L., Garcia,E., Martinez,L.M., FLORES,N., Buchs,J., Gosset,G., Lara,A.R. (2024). Sigma factors as potential targets to enhance recombinant protein expression.
bioRxiv,
Preprint posted November 12.
52 - Flores Mejia, Noemi (2005). Analisis del transcriptoma en cepas de Escherichia coli que carecen del sistema de fosfotransferasa (PTS) y su comparacion con la progenitora silvestre .
Instituto de Biotecnologia,
Doctoado en Ciencias Bioquimicas,
UNAM.
Asesor: Bolivar Zapata, Francisco
53 - Flores Mejia, Noemi (1995). Construccion y caracterizacion de cepas de Escherichia coli mutantes en el sistema de transporte de carbohidratos PTS.
Instituto de Biotecnologia,
Maestria en Biotecnologia,
UNAM.
Asesor: Bolivar Zapata, Francisco
54 - Flores Mejia, Noemi (1985). Un nuevo vector de clonacion para porducir proteinas hibridas en escherichia coli .
Facultad de Ciencias,
Licenciatura en Biología,
UNAM.
*
Asesor: Valle Baheza, Fernando
Artículo in press communication
55 - Pohlen,L., Garcia,E., Martinez,L.M., FLORES,N., Buchs,J., Gosset,G., Lara,A.R.. Sigma factors as potential targets to enhance recombinant protein expression.
Biotechnology and Bioengineering,
Feb 24 [Online ahead of print].
Artículo in press
56 - Vallejo-Giraldo,A., Martinez,L.M., FLORES,N., Hernandez-Chavez,G., Cueto,F., Martinez,A., Corona-Gonzalez,R.I., Gosset,G.. Construction and Characterization of Novel Shuttle Expression Vectors for Actinobacillus succinogenes.
Biotechnology and Applied Biochemistry,
Feb 17 [Online ahead of print].
Artículo
57 - Escalante,A., Astudillo,F., Larios,V., FLORES,N., Gosset,G., Bolivar,F.. The complete genome of Escherichia coli JM101 assembled with a combination of Nanopore and Illumina platforms.
Microbiology Resource Announcements,
13 (2),
e0097323.
Artículo
58 - Soria,S., Carreon-Rodriguez,O.E., de Anda,R., FLORES,N., Escalante,A., Bolivar,F.. Transcriptional and Metabolic Response of a Strain of Escherichia coli PTS(-) to a Perturbation of the Energetic Level by Modification of [ATP]/[ADP] Ratio.
BioTech (Basel),
13 (2),
10.
Preprint
59 - Pohlen,L., Garcia,E., Martinez,L.M., FLORES,N., Buchs,J., Gosset,G., Lara,A.R.. Sigma factors as potential targets to enhance recombinant protein expression.
bioRxiv,
Preprint posted November 12.
Artículo
60 - Fragoso-Jimenez,J.C., Gutierrez-Rios,R.M., FLORES,N., Martinez,A., Lara,A.R., Delvigne,F., Gosset,G.. Glucose consumption rate-dependent transcriptome profiling of Escherichia coli provides insight on performance as microbial factories.
Microbial Cell Factories,
21 (1),
189.
Artículo
61 - Rodriguez-Ochoa,J.I., Fragoso-Jimenez,J.C., FLORES,N., Martinez,L.M., Bolivar,F., Martinez,A., Gosset,G.. Global transcriptomic response of Escherichia coli to p-coumaric acid.
Microbial Cell Factories,
21 (1),
148.
Artículo
62 - Carmona,S.B., FLORES,N., Martinez-Romero,E., Gosset,G., Bolivar,F., Escalante,A.. Evolution of an Escherichia coli PTS(-) strain: a study of reproducibility and dynamics of an adaptive evolutive process.
Applied Microbiology and Biotechnology,
104,
9309?9325.
Artículo
63 - Cristiano-Fajardo,S.A., Flores,C., FLORES,N., Tinoco-Valencia,R., Serrano-Carreon,L., Galindo,E.. Glucose limitation and glucose uptake rate determines metabolite production and sporulation in high cell density continuous cultures of Bacillus amyloliquefaciens 83.
Journal of Biotechnology,
299,
57-65.
Artículo
64 - Aguilar,C., Martinez-Batallar,G., FLORES,N., Moreno-Avitia,F., Encarnacion,S., Escalante,A., Bolivar,F.. Analysis of differentially upregulated proteins in ptsHIcrr(-) and rppH(-) mutants in Escherichia coli during an adaptive laboratory evolution experiment.
Applied Microbiology and Biotechnology,
102 (23),
10193?10208 .
Artículo
65 - Martinez,J.A., Rodriguez,A., Moreno,F., FLORES,N., Lara,A.R., Ramirez,O.T., Gosset,G., Bolivar,F.. Metabolic modeling and response surface analysis of an Escherichia coli strain engineered for shikimic acid production.
BMC Systems Biology,
12 (1),
102.
Artículo
66 - Salas-Navarrete, C., Hernandez-Chavez, G., Flores, N., Martinez, L.M., Martinez, A., Bolivar, F., Barona-Gomez, F., Gosset, G.. Increasing pinosylvin production in Escherichia coli by reducing the expression level of the gene fabI-encoded enoyl-acyl carrier protein reductase.
Electronic Journal of Biotechnology,
33,
11-16.
Artículo
67 - Garcia, S., Flores, N., de Anda, R., Hernandez, G., Gosset, G., Bolivar, F., Escalante, A.. The Role of the ydiB Gene, Which Encodes Quinate/Shikimate Dehydrogenase, in the Production of Quinic, Dehydroshikimic and Shikimic Acids in a PTS- Strain of Escherichia coli.
Journal of Molecular Microbiology and Biotechnology,
27 (1),
11-21.
Artículo
68 - Cortes,J.T., FLORES,N., Bolivar,F., Lara,A.R., Ramirez,O.T.. Physiological effects of pH gradients on Escherichia coli during plasmid DNA production.
Biotechnology and Bioengineering,
113 (3),
598-611.
Artículo
69 - Fernandez-Alejandre,K.I., FLORES,N., Tinoco-Valencia,R., Caro,M., Flores,C., Galindo,E., Serrano-Carreon,L.. Diffusional and transcriptional mechanisms involved in laccases production by Pleurotus ostreatus CP50.
Journal of Biotechnology,
223,
42-49.
Artículo
70 - Aguilar,C., FLORES,N., Riveros-McKay,F., Sahonero-Canavesi,D., Carmona,S.B., Geiger,O., Escalante,A., Bolivar,F.. Deletion of the 2-acyl-glycerophosphoethanolamine cycle improve glucose metabolism in Escherichia coli strains employed for overproduction of aromatic compounds.
Microbial Cell Factories,
14 (1),
194.
Revisión
71 - Rodriguez,A., Martinez,J.A., FLORES,N., Escalante,A., Gosset,G., Bolivar,F.. Engineering Escherichia coli to overproduce aromatic amino acids and derived compounds.
Microbial Cell Factories,
13 (1),
126.
Artículo
72 - Sabido,A., Sigala,J.C., Hernandez-Chavez,G., FLORES,N., Gosset,G., Bolivar,F.. Physiological and transcriptional characterization of Escherichia coli strains lacking interconversion of phosphoenolpyruvate and pyruvate when glucose and acetate are coutilized.
Biotechnology and Bioengineering,
111 (6),
1150-1160.
Artículo
73 - Caspeta,L., Lara,A.R., Perez,N.O., FLORES,N., Bolivar,F., Ramirez,O.T.. Enhancing thermo-induced recombinant protein production in Escherichia coli by temperature oscillations and post-induction nutrient feeding strategies.
Journal of Biotechnology,
167 (1),
47-55.
Artículo
74 - Centeno-Leija,S., Utrilla,J., FLORES,N., Rodriguez,A., Gosset,G., Martinez,A.. Metabolic and transcriptional response of Escherichia coli with a NADP-dependent glyceraldehyde 3-phosphate dehydrogenase from Streptococcus mutans.
Antonie Van Leeuwenhoek,
104 (6),
913-924.
Artículo
75 - Rodriguez,A., Martinez,J.A., Baez-Viveros,J.L., FLORES,N., Hernandez-Chavez,G., Ramirez,O.T., Gosset,G., Bolivar,F.. Constitutive expression of selected genes from the pentose phosphate and aromatic pathways increases the shikimic acid yield in high-glucose batch cultures of an Escherichia coli strain lacking PTS and pykF.
Microbial Cell Factories,
12 (1),
86.
Artículo
76 - Soria,S., de Anda,R., FLORES,N., Romero-Garcia,S., Gosset,G., Bolivar,F., Baez-Viveros,J.L.. New insights on transcriptional responses of genes involved in carbon central metabolism, respiration and fermentation to low ATP levels in Escherichia coli.
Journal of Basic Microbiology,
53 (4),
365-380.
Artículo
77 - Aguilar,C., Escalante,A., FLORES,N., de Anda,R., Riveros-McKay,F., Gosset,G., Morett,E., Bolivar,F.. Genetic changes during a laboratory adaptive evolution process that allowed fast growth in glucose to an Escherichia coli strain lacking the major glucose transport system.
BMC Genomics,
13 (1),
385.
Artículo
78 - Cabrera-Valladares,N., Martinez,L.M., FLORES,N., Hernandez-Chavez,G., Martinez,A., Bolivar,F., Gosset,G.. Physiologic Consequences of Glucose Transport and Phosphoenolpyruvate Node Modifications in Bacillus subtilis 168.
Journal of Molecular Microbiology and Biotechnology,
22 (3),
177-197.
Artículo
79 - Martinez-Gomez,K., FLORES,N., Castaneda,H.M., Martinez-Batallar,G., Hernandez-Chavez,G., Ramirez,O.T., Gosset,G., Encarnacion,S., Bolivar,F.. New insights into Escherichia coli metabolism: carbon scavenging, acetate metabolism and carbon recycling responses during growth on glycerol.
Microbial Cell Factories,
11 (1),
46.
Artículo
80 - Baez,A., FLORES,N., Bolivar,F., Ramirez,O.T.. Simulation of dissolved CO(2) gradients in a scale-down system: A metabolic and transcriptional study of recombinant Escherichia coli.
Biotechnology Journal,
6 (8),
959-967.
Artículo
81 - Baez,A., FLORES,N., Bolivar,F., Ramirez,O.T.. Metabolic and transcriptional response of recombinant Escherichia coli to elevated dissolved carbon dioxide concentrations.
Biotechnology and Bioengineering,
104 (1),
102-110.
Artículo
82 - Caspeta,L., FLORES,N., Perez,N.O., Bolivar,F., Ramirez,O.T.. The effect of heating rate on Escherichia coli metabolism, physiological stress, transcriptional response, and production of temperature-induced recombinant protein: a scale-down study.
Biotechnology and Bioengineering,
102 (2),
468-482.
Artículo
83 - Olvera,L., Mendoza-Vargas,A., FLORES,N., Olvera,M., Sigala,J.C., Gosset,G., Morett,E., Bolivar,F.. Transcription Analysis of Central Metabolism Genes in Escherichia coli. Possible Roles of a38 in Their Expression, as a Response to Carbon Limitation.
PLoS ONE,
4 (10),
e7466.
Artículo
84 - Sigala,J.C., Flores,S., FLORES,N., Aguilar,C., de Anda,R., Gosset,G., Bolivar,F.. Acetate Metabolism in Escherichia coli Strains Lacking Phosphoenolpyruvate: Carbohydrate Phosphotransferase System; Evidence of Carbon Recycling Strategies and Futile Cycles.
Journal of Molecular Microbiology and Biotechnology,
16 (3-4),
224-235.
Artículo
85 - FLORES,N., Escalante,A., de Anda,R., Baez-Viveros,J.L., Merino,E., Franco,B., Georgellis,D., Gosset,G., Bolivar,F.. New Insights into the Role of Sigma Factor RpoS as Revealed in Escherichia coli Strains Lacking the Phosphoenolpyruvate:Carbohydrate Phosphotransferase System.
Journal of Molecular Microbiology and Biotechnology,
14 (4),
176-192.
Artículo
86 - Orencio-Trejo,M., FLORES,N., Escalante,A., Hernandez-Chavez,G., Bolivar,F., Gosset,G., Martinez,A.. Metabolic regulation analysis of an ethanologenic Escherichia coli strain based on RT-PCR and enzymatic activities.
Biotechnology for Biofuels,
1 (1),
8.
Artículo
87 - Baez-Viveros,J.L., FLORES,N., Suarez,K., Castillo-Espana,P., Bolivar,F., Gosset,G.. Metabolic transcription analysis of engineered Escherichia coli strains that overproduce L-phenylalanine.
Microbial Cell Factories,
6 (1),
30.
Artículo
88 - FLORES,N., Leal,L., Sigala,J.C., de Anda,R., Escalante,A., Martinez,A., Ramirez,O.T., Gosset,G., Bolivar,F.. Growth Recovery on Glucose under Aerobic Conditions of an Escherichia coli Strain Carrying a Phosphoenolpyruvate:Carbohydrate Phosphotransferase System Deletion by Inactivating arcA and Overexpressing the Genes Coding for Glucokinase and Galactose Permease.
Journal of Molecular Microbiology and Biotechnology,
13 (1-3),
105-116.
Artículo
89 - Lara,A.R., Leal,L., FLORES,N., Gosset,G., Bolivar,F., Ramirez,O.T.. Transcriptional and metabolic response of recombinant Escherichia coli to spatial dissolved oxygen tension gradients simulated in a scale-down system.
Biotechnology and Bioengineering,
93 (2),
372-385.
Artículo
90 - FLORES,N., Flores,S., Escalante,A., de Anda,R., Leal,L., Malpica,R., Georgellis,D., Gosset,G., Bolivar,F.. Adaptation for fast growth on glucose by differential expression of central carbon metabolism and gal regulon genes in an Escherichia coli strain lacking the phosphoenolpyruvate:carbohydrate phosphotransferase system.
Metabolic Engineering,
7 (2),
70-87.
Artículo
91 - Flores,S., FLORES,N., de Anda,R., Gonzalez,A., Escalante,A., Sigala,J.C., Gosset,G., Bolivar,F.. Nutrient-Scavenging Stress Response in an Escherichia coli Strain Lacking the Phosphoenolpyruvate:Carbohydrate Phosphotransferase System, as Explored by Gene Expression Profile Analysis.
Journal of Molecular Microbiology and Biotechnology,
10 (1),
51-63.
92 - Flores Mejia, Noemi. Analisis del transcriptoma en cepas de Escherichia coli que carecen del sistema de fosfotransferasa (PTS) y su comparacion con la progenitora silvestre .
Instituto de Biotecnologia,
Doctoado en Ciencias Bioquimicas,
UNAM.
Asesor: Valle Baheza, Fernando
Artículo
93 - FLORES,N., de Anda,R., Flores,S., Escalante,A., Hernandez,G., Martinez,A., Ramirez,O.T., Gosset,G., Bolivar,F.. Role of Pyruvate Oxidase in Escherichia coli Strains Lacking the Phosphoenolpyruvate:Carbohydrate Phosphotransferase System.
Journal of Molecular Microbiology and Biotechnology,
8 (4),
209-221.
Artículo
94 - Flores,S., Gosset,G., FLORES,N., de Graaf,A.A., Bolivar,F.. Analysis of carbon metabolism in Escherichia coli strains with an inactive phosphotransferase system by 13C labelling and NMR spectroscopy.
Metabolic Engineering,
4 (2),
124-137.
Artículo
95 - Siguenza,R., FLORES,N., Hernandez,G., Martinez,A., Bolivar,F., Valle,F.. Kinetic characterization in batch and continuous culture of Escherichia coli mutants affected in phosphoenolpyruvate metabolism: differences in acetic acid production.
World Journal of Microbiology & Biotechnology,
15 (5),
587-592.
Artículo
96 - FLORES,N., Xiao,J., Berry,A., Bolivar,F., Valle,F.. Pathway engineering for the production of aromatic compounds in Escherichia coli.
Nature Biotechnology,
14 (5),
620-623.
Artículo
97 - Valle,F., Munoz,E., Ponce,E., FLORES,N., Bolivar,F.. Basic and applied aspects of metabolic diversity: The phosphoenolpyruvate node.
Journal of Industrial Microbiology & Biotechnology,
17 (5-6),
458-462.
Note
98 - Ponce,E., FLORES,N., Martinez,A., Valle,F., Bolivar,F.. Cloning of the two pyruvate kinase isoenzyme structural genes from Escherichia coli: the relative roles of these enzymes in pyruvate biosynthesis.
Journal of Bacteriology,
177 (19),
5719-5722.
99 - Flores Mejia, Noemi. Construccion y caracterizacion de cepas de Escherichia coli mutantes en el sistema de transporte de carbohidratos PTS.
Instituto de Biotecnologia,
Maestria en Biotecnologia,
UNAM.
Asesor: Valle Baheza, Fernando
Artículo
100 - Castano,I., FLORES,N., Valle,F., Covarrubias,A.A., Bolivar,F.. gltF, a member of the gltBDF operon of Escherichia coli, is involved in nitrogen-regulated gene expression.
Molecular Microbiology,
6 (18),
2733-2741.
Artículo
101 - FLORES,N., Valle,F., Bolivar,F., Merino,E.. Recovery of DNA from agarose gels stained with methylene blue.
Biotechniques,
13 (2),
203-205.
Memoria in extenso
102 - Balbas,P., de Anda,R., FLORES,N., Alvarado,X., Cruz,N., Valle,F., Bolivar,F.. Overproduction of proteins by recombinant DNA: human insulin. Cell Function and Disease.
59-74,
New York.
Artículo
103 - Oliver,G., Gosset,G., Sanchez-Pescador,R., Lozoya,E., Ku,L.M., FLORES,N., Becerril,B., Valle,F., Bolivar,F.. Determination of the nucleotide sequence for the glutamate synthase structural genes of Escherichia coli K-12.
Gene,
60 (1),
1-11.
Artículo
104 - Balbas,P., Soberon,X., Merino,E., Zurita,M., Lomeli,H., Valle,F., FLORES,N., Bolivar,F.. Plasmid vector pBR322 and its special-purpose derivatives--a review.
Gene,
50 (1-3),
3-40.
Artículo
105 - FLORES,N., de Anda,R., Guereca,L., Cruz,N., Antonio,S., Balbas,P., Bolivar,F., Valle,F.. A new expression vector for the production of fused proteins in Escherichia coli.
Applied Microbiology and Biotechnology,
25 (3),
267-271.
106 - Flores Mejia, Noemi. Un nuevo vector de clonacion para porducir proteinas hibridas en escherichia coli .
Facultad de Ciencias,
Licenciatura en Biología,
UNAM.
*
Asesor: Valle Baheza, Fernando