Dr. Takuya Nishigaki
Investigador Titular B Tiempo Completo
Desarrollo de biosensores
Fisiología molecular del espermatozoide
Número de registros: 13
- Activos
- Vencidos
- Estudio de interacciones moleculares mediante proteínas fluorescentes y caracterización de la adenilil ciclasa soluble, DIRECCIÓN GENERAL DE ASUNTOS DEL PERSONAL ACADÉMICO/UNIVERSIDAD NACIONAL AUTÓNOMA DE MÉXICO, 2025 - 2027
- Estudio de interacciones moleculares específicas usando proteínas fluorescentes, DIRECCIÓN GENERAL DE ASUNTOS DEL PERSONAL ACADÉMICO/UNIVERSIDAD NACIONAL AUTÓNOMA DE MÉXICO, 2022 - 2024
- Caracterización funcional del intercambiador na+/h+ específico del espermatozoide, CONSEJO NACIONAL DE CIENCIA Y TECNOLOGÍA, 2019 - 2022
- Mecanismo de regulación del ph citosólico del espermatozoide mediante el intercambiador na+/h+ y un canal de protón voltaje dependiente., DIRECCIÓN GENERAL DE ASUNTOS DEL PERSONAL ACADÉMICO/UNIVERSIDAD NACIONAL AUTÓNOMA DE MÉXICO, 2019 - 2021
- Acoplamiento funcional entre intercambiador na+/h+específico al espermatozoide (snhe) y canal de ca2+ específico alespermatozaoide (catsper) en mamíferos, UNIVERSIDAD DE CALIFORNIA-MÉXICO, 2016 - 2018
- Estudio de tres proteínas específicas del espermatozoide, catsper, snhe y sac en la regulación del batido flagelar, DIRECCIÓN GENERAL DE ASUNTOS DEL PERSONAL ACADÉMICO/UNIVERSIDAD NACIONAL AUTÓNOMA DE MÉXICO, 2016 - 2019
- Estudio de la regulación de la motilidad del espermatozoide por medio de herramientas ópticas, PRESUPUESTO UNAM, 2014 - 2020
- Mecanismo de regulación de la movilidad de los espermatozoides de mamíferos mediante ca2+, ph y amp cíclico, DIRECCIÓN GENERAL DE ASUNTOS DEL PERSONAL ACADÉMICO/UNIVERSIDAD NACIONAL AUTÓNOMA DE MÉXICO, 2013 - 2016
- Estudio de la regulación de la movilidad del espermatozoide por ca2+, ph y amp cíclico usando herramientas ópticas, CONSEJO NACIONAL DE CIENCIA Y TECNOLOGÍA, 2012 - 2016
- Efectos de progesterona y prostaglandina e1 en la movilidad del espermatozoide de mamífero, DIRECCIÓN GENERAL DE ASUNTOS DEL PERSONAL ACADÉMICO/UNIVERSIDAD NACIONAL AUTÓNOMA DE MÉXICO, 2010 - 2012
- Desarrollo de un sensor de fluorescencia para amp ciclico, CONSEJO NACIONAL DE CIENCIA Y TECNOLOGÍA, 2009
- Determinación de los cambios de ampc en el espermatozoide en tiempo real usando un sensor fluorescente, CONSEJO NACIONAL DE CIENCIA Y TECNOLOGÍA, 2007 - 2010
- Determinación cinética de los cambios de ampc en el espermatozoide usando un sensor fluorescente, DIRECCIÓN GENERAL DE ASUNTOS DEL PERSONAL ACADÉMICO/UNIVERSIDAD NACIONAL AUTÓNOMA DE MÉXICO, 2007 - 2009
Nivel C del PRIDE
Nivel III del SNII
Nivel III del SNII
Doctorado, en Biociencias, Instituto Tecnologico de Tokyo (1997)
Maestría, en Biociencias, Instituto Tecnologico de Tokyo (1994)
Licenciatura, Ciencias, Instituto Tecnologico de Tokyo (1992)
- Activos
- Graduados
-
Doctorado
-
Maestría
-
Licenciatura
-
Estancia Temporal
1 - Sanchez Guevara, Yoloxochitl (2024). Determinación semicuantitativa de niveles de calcio mitocondrial en espermatozoides de ratón usando indicadores fluorescentes .
Instituto de Biotecnologia,
Doctorado en Ciencias Biomédicas,
UNAM.
Asesor: Nishigaki Shimizu, Takuya
2 - Ruíz Rodríguez, Kathia Jasmin Janet (2024). Evaluación de nuevos pares de FRET entre dos proteínas fluorescentes; usando un donador con desplazamiento de Stokes largo.
Licenciatura Ingenieria en Biotecnología,
Universidad Politecnica de Estado de Morelos.
*
Asesor: Nishigaki Shimizu, Takuya
3 - Hernandez Garduno, Sandra (2023). Estudio de la actividad del intercambiador Na+/H+ especifico del espermatozoide en mamiferos.
Instituto de Biotecnologia,
Doctorado en Ciencias Biomedicas,
UNAM.
Asesor: Nishigaki Shimizu, Takuya
4 - Arcos Hernandez, Cesar (2023). Estudio funcional del dominio sensor de voltaje del intercambiador Na+/H+ especifico de espermatozoide de mamifero.
Instituto de Biotecnologia,
Doctorado en Bioquimicas,
UNAM.
Asesor: Nishigaki Shimizu, Takuya
5 - López Velarde, Columba Alejandra (2020). Ensayo de unión tipo FRET in vivo entre un dominio de unión a nucleótidos cíclicos y AMPc .
Facultad de Ciencias,
Licenciatura en Biología,
UNAM.
*
Asesor: Nishigaki Shimizu, Takuya
6 - Romero Corpus, Francisco (2018). Estudio bioquimico y filogenetico del intercambiador Na+/H+ especifico del espermatozoide .
Instituto de Biotecnologia,
Doctorado en Bioquimicas,
UNAM.
Asesor: Nishigaki Shimizu, Takuya
7 - Santana Calvo, Maria del Carmen (2016). Optimizacion del ensayo de union tipo FRET entre un dominio de union a nucleotidos ciclicos y AMPc .
Instituto de Biotecnologia,
Maestria en Ciencias Bioquimicas,
UNAM.
Asesor: Nishigaki Shimizu, Takuya
8 - Monroy Lara, Juan Esteban (2016). Caracterizacion espectroscopica de proteinas quimericas construidas a partir de una proteina fluorescente y de un dominio de union a nucleotidos ciclocos : un intento para desarrollar un nuevo sensor fluorescente para el AMPC.
Instituto de Biotecnologia,
Maestria en Ciencias Bioquimicas,
UNAM.
Asesor: Nishigaki Shimizu, Takuya
9 - Santana Calvo, Maria del Carmen (2015). Desarrollo de un ensayo de union tipo fret entre un dominio de union a nucleotidos ciclicos y AMPc.
Facultad de Ciencias,
Licenciatura en Biologia,
UNAM.
*
Asesor: Nishigaki Shimizu, Takuya
10 - Romero Corpus, Francisco (2012). Caracterizacion bioquimica del dominio de union a nucleotidos ciclicos del intercambiador na+/h+ especifico para espermatozoide de mamifero.
Instituto de Biotecnologia,
Maestria en Ciencias Bioquimicas,
UNAM.
Asesor: Nishigaki Shimizu, Takuya
11 - Servin Vences, Martha Rocio (2011). Cambios en la concentracion del Ca2+ intracelular y en la movilidad del espermatozoide de humano inducidos por progesterona en fluidos de alta viscosidad.
Instituto de Biotecnologia,
Maestria en Ciencias Bioquimicas,
UNAM.
Asesor: Nishigaki Shimizu, Takuya
12 - Hernandez Rueda, Yadira Libertad (2011). Participacion del canal CatSper en el incremento de calcio intracelular inducido por PGE1 y progesterona en espermatozoides humanos .
Facultad de Ciencias,
Maestria en Ciencias Biologicas,
UNAM.
*
Asesor: Nishigaki Shimizu, Takuya
13 - Servin Vences, Martha Rocio (2009). Evaluacion de tres analogos fotoactivables de progesterona en la respuesta de la Ca2+ i en espermatozoide de humano .
Facultad de Ciencias,
Licenciatura en Biología,
UNAM.
*
Asesor: Nishigaki Shimizu, Takuya
Total de publicaciones: 63
- Tipo
- Fecha
Artículo
14 - Arevalo-Salina,E.L., Nishigaki,T., Olvera,L., Gonzalez-Andrade,M., Xolalpa-Villanueva,W., Lopez-Romero,E.O., Soberon,X., Saab-Rincon,G. (2025). Change in selectivity of estrogen receptor alpha ligand-binding domain by mutations at residues H524/L525.
Biochimica et Biophysica Acta.General Subjects,
1869 (4),
130775.
Artículo
15 - Lopez-Romero,E.O., Arevalo-Salina,E.L., Arcos-Hernandez,C., Sanchez-Guevara,Y., Beltran,C., Saab-Rincon,G., Nishigaki,T. (2025). A FRET-based competitive binding assay using coumestrol and the ligand-binding domain of human estrogen receptor alpha tagged with mTurquoise2 efficiently expressed in E. coli with ethanol.
Protein Expression and Purification,
229,
106667.
Revisión
16 - Chavez,J.C., Carrasquel-Martinez,G., Hernandez-Garduno,S., Matamoros-Volante,A., Trevino,C.L., Nishigaki,T., Darszon,A. (2024). Cytosolic and Acrosomal pH Regulation in Mammalian Sperm.
Cells,
13 (10),
865.
Artículo
17 - Novero,A.G., Torres-Rodriguez,P., de la Vega-Beltran,J.L., Schiavi-Ehrenhaus,L.J., Luque,G.M., Carruba,M., Stival,C., Gentile,I., Ritagliati,C., Santi,C.M., Nishigaki,T., Krapf,D., Buffone,M.G., Darszon,A., Trevino,C.L., Krapf,D. (2024). The sodium-proton exchangers sNHE and NHE1 control plasma membrane hyperpolarization in mouse sperm.
Journal of Biological Chemistry,
300 (12),
107932.
Artículo
18 - Pacheco-Castillo,H., Zagal-Huerta,E.E., Acevedo-Fernandez,J.J., Negrete-Leon,E., Nishigaki,T., Beltran,C. (2024). Hyperglycemia adversely affects critical physiological events related to rat sperm capacitation.
Biochemical and Biophysical Research Communications,
734,
150610.
Artículo
19 - Sanchez-Guevara,Y., Oliver,E.I., Nishigaki,T. (2024). Ca2+ concentrations in mouse sperm mitochondria fluctuate according to the cytosol.
Reproduction,
167 (2),
e230237.
Artículo
20 - Arcos-Hernandez,C., Suarez-Delgado,E., Islas,L.D., Romero,F., Lopez-Gonzalez,I., Ai,H.W., Nishigaki,T. (2023). How to study a highly toxic protein to bacteria: A case of voltage sensor domain of mouse sperm-specific sodium/proton exchanger.
Protein Expression and Purification,
201,
106172.
Revisión
21 - Arcos-Hernandez,C., Nishigaki,T. (2023). Ion currents through the voltage sensor domain of distinct families of proteins.
Journal of Biological Physics,
49,
393-413.
Artículo
22 - Hernandez-Garduno,S., Chavez,J.C., Matamoros-Volante,A., Sanchez-Guevara,Y., Torres,P., Trevino,C.L., Nishigaki,T. (2022). Hyperpolarization induces cytosolic alkalization of mouse sperm flagellum probably through sperm Na+/H+ exchanger.
Reproduction,
164 (4),
125-134.
Artículo
23 - Chavez,J.C., Darszon,A., Trevino,C.L., Nishigaki,T. (2020). Quantitative intracellular pH determinations in single live mammalian spermatozoa using the ratiometric dye SNARF-5F.
Frontiers in Cell and Developmental Biology,
7,
366.
Revisión
24 - Darszon,A., Nishigaki,T., Lopez-Gonzalez,I., Visconti,P.E., Trevino,C.L. (2020). Differences and Similarities: The Richness of Comparative Sperm Physiology.
Physiology (Bethesda.),
35 (3),
196-208.
Artículo
25 - De La Rosa,J.A.M., Garcia-Castaneda,M., Nishigaki,T., Gomora,J.C., Mancilla-Percino,T., Avila,G. (2020). Interaction of MDIMP with the voltage-gated calcium channels.
Molecular Pharmacology,
98 (3),
211-221.
Artículo
26 - Garza-Lopez,E., Aldana,A., Darszon,A., Nishigaki,T., Lopez-Gonzalez,I. (2020). CaV3.1 channel pore pseudo-symmetry revealed by selectivity filter mutations in its domains I/II.
Cell Calcium,
89,
102214.
Artículo
27 - Priego-Espinosa,D.A., Darszon,A., Guerrero,A., Gonzalez-Cota,A.L., Nishigaki,T., Martinez-Mekler,G., Carneiro,J. (2020). Modular analysis of the control of flagellar Ca2+-spike trains produced by CatSper and CaV channels in sea urchin sperm.
PLoS Computational Biology,
16 (3),
e1007605.
Artículo
28 - Kinoshita-Terauchi, N., Shiba, K., Terauchi, M., Romero, F., Ramirez-Gomez, H. V., Yoshida, M., Motomura, T., Kawai, H., Nishigaki, T. (2019). High potassium seawater inhibits ascidian sperm chemotaxis, but does not affect the male gamete chemotaxis of a brown alga.
Zygote,
27 (4),
225-231.
Artículo
Artículo
30 - Chavez, J.C., De la Vega-Beltran JL, Jose, O., Torres, P., Nishigaki, T., Trevino, C.L., Darszon, A. (2018). Acrosomal alkalization triggers Ca2+ release and acrosome reaction in mammalian spermatozoa.
Journal of Cellular Physiology,
233 (6),
4735-4747.
Artículo
31 - Santana-Calvo, C., Romero, F., Lopez-Gonzalez, I., Nishigaki, T. (2018). Robust evaluation of intermolecular FRET using a large Stokes shift fluorophore as a donor.
Biotechniques,
65 (4),
211-218.
Artículo
32 - Romero, F., Santana-Calvo, C., Sanchez-Guevara, Y., Nishigaki, T. (2017). FRET-based binding assay between a fluorescent cAMP analogue and a cyclic nucleotide-binding domain tagged with a CFP.
FEBS Letters,
591 (18),
2869-2878.
Artículo
33 - Arcos-Hernandez,C., Romero,F., Sanchez-Guevara,Y., Beltran,C., Nishigaki,T. (2016). FRET analysis using sperm-activating peptides tagged with fluorescent proteins reveals that ligand-binding sites exist as clusters.
Journal of Experimental Biology,
219 (Pt 4),
508-515.
Artículo
34 - Garza-Lopez,E., Chavez,J.C., Santana-Calvo,C., Lopez-Gonzalez,I., Nishigaki,T. (2016). Cd2+ sensitivity and permeability of a low voltage-activated Ca2+ channel with CatSper-like selectivity filter.
Cell Calcium,
60 (1),
41-50.
Artículo
35 - Gijsbers,A., Nishigaki,T., Sanchez-Puig,N. (2016). Fluorescence Anisotropy as a Tool to Study Protein-protein Interactions.
Journal of Visualized Experiments JoVE,
116.
Artículo
36 - Nishigaki,T., Romero,F., Sanchez-Guevara,Y. (2016). CatSper, el canal de Ca2+ que regula el batido flagelar del espermatozoide en eucariontes.
Revista Iberoamericana de Ciencias,
3 (3),
28-36.
Artículo
37 - Garza-Lopez,E., Sanchez-Carranza,O., Nishigaki,T., Lopez-Gonzalez,I. (2015). Pharmacological identification of endogenous slo1 channel-á1 subunit complexes in CHO cells using three àKTx1 subfamily toxins.
International Journal of Pharmacy & Therapeutics,
6 (1),
11-24.
Revisión
38 - Beltran,C., Rodriguez-Miranda,E., Granados-Gonzalez,G., de De la Torre LG, Nishigaki,T., Darszon,A. (2014). Zn induces hyperpolarization by activation of a K channel and increases intracellular Ca and pH in sea urchin spermatozoa.
Developmental Biology,
394 (1),
15-23.
Revisión
39 - Nishigaki,T., Jose,O., Gonzalez-Cota,A.L., Romero,F., Trevino,C.L., Darszon,A. (2014). Intracellular pH in Sperm Physiology.
Biochemical and Biophysical Research Communications,
450 (3),
1149-1158.
Artículo
40 - Sanchez-Tusie,A.A., Vasudevan,S.R., Churchill,G.C., Nishigaki,T., Trevino,C.L. (2014). Characterization of NAADP-Mediated Calcium Signaling in Human Spermatozoa.
Biochemical and Biophysical Research Communications,
443 (2),
531-536.
Artículo
41 - Servin-Vences,M.R., Tatsu,Y., Ando,H., Guerrero,A., Yumoto,N., Darszon,A., Nishigaki,T. (2012). A caged progesterone analog alters intracellular Ca2+ and flagellar bending in human sperm.
Reproduction,
144 (1),
101-109.
Artículo
42 - Chavez,J.C., de Blas,G.A., de la Vega-Beltran,J.L., Nishigaki,T., Chirinos,M., Gonzalez-Gonzalez,M.E., Larrea,F., Solis,A., Darszon,A., Trevino,C.L. (2011). The opening of maitotoxin-sensitive calcium channels induces the acrosome reaction in human spermatozoa: differences from the zona pellucida.
Asian Journal of Andrology,
13,
159-165.
Revisión
43 - Darszon,A., Nishigaki,T., Beltran,C., Trevino,C.L. (2011). Calcium Channels in the Development, Maturation, and Function of Spermatozoa.
Physiological Reviews,
91 (4),
1305-1355.
Artículo
44 - Guerrero,A., Wood,C.D., Nishigaki,T., Carneiro,J., Darszon,A. (2010). Tuning sperm chemotaxis.
Biochemical Society Transactions,
38 (5),
1270-1274.
Artículo
45 - Guerrero,A., Nishigaki,T., Carneiro,J., Tatsu,Y., Wood,C.D., Darszon,A. (2010). Tuning sperm chemotaxis by calcium burst timing.
Developmental Biology,
344 (1),
52-65.
Artículo
46 - Darszon,A., Guerrero,A., Galindo,B.E., Nishigaki,T., Wood,C.D. (2008). Sperm-activating peptides in the regulation of ion fluxes, signal transduction and motility.
International Journal of Developmental Biology,
52 (5-6),
595-606.
Artículo
47 - de la Sancha,C.U., Martinez-Cadena,G., Lopez-Godinez,J., Castellano,L.E., Nishigaki,T., Darszon,A., Garcia-Soto,J. (2007). Rho-kinase (ROCK) in sea urchin sperm: Its role in regulating the intracellular pH during the acrosome reaction.
Biochemical and Biophysical Research Communications,
364 (3),
470-475 [Corrigendum en Volume 366, Issue 2, 2008, Page 604].
Artículo
48 - Rangel-Mata,F., Mendez-Marquez,R., Martinez-Cadena,G., Lopez-Godinez,J., Nishigaki,T., Darszon,A., Garcia-Soto,J. (2007). Rho, Rho-kinase, and the actin cytoskeleton regulate the Na(+)-H(+) exchanger in sea urchin eggs.
Biochemical and Biophysical Research Communications,
352 (1),
264-269.
Artículo
49 - Wood,C.D., Nishigaki,T., Tatsu,Y., Yumoto,N., Baba,S.A., Whitaker,M., Darszon,A. (2007). Altering the speract-induced ion permeability changes that generate flagellar Ca(2+) spikes regulates their kinetics and sea urchin sperm motility.
Developmental Biology,
306 (2),
525-537.
Revisión
50 - Darszon,A., Acevedo,J.J., Galindo,B.E., Hernandez-Gonzalez,E.O., Nishigaki,T., Trevino,C.L., Wood,C., Beltran,C. (2006). Sperm channel diversity and functional multiplicity.
Reproduction,
131 (6),
977-988.
Artículo
51 - Nishigaki,T., Wood,C.D., Shiba,K., Baba,S.A., Darszon,A. (2006). Stroboscopic illumination using light-emitting diodes reduces phototoxicity in fluorescence cell imaging.
Biotechniques,
41 (2),
191-197.
Artículo
52 - Trevino,C.L., de la Vega-Beltran,J.L., Nishigaki,T., Felix,R., Darszon,A. (2006). Maitotoxin potently promotes Ca(2+) influx in mouse spermatogenic cells and sperm, and induces the acrosome reaction.
Journal of Cellular Physiology,
206 (2),
449-456.
Revisión
53 - Darszon,A., Nishigaki,T., Wood,C., Trevino,C.L., Felix,R., Beltran,C. (2005). Calcium channels and ca(2+) fluctuations in sperm physiology.
International Review of Cytology,
243,
79-172.
Artículo
54 - Shiba,K., Ohmuro,J., Mogami,Y., Nishigaki,T., Wood,C.D., Darszon,A., Tatsu,Y., Yumoto,N., Baba,S.A. (2005). Sperm-activating Peptide induces asymmetric flagellar bending in sea urchin sperm.
Zoological Science,
22 (3),
293-299.
Artículo
55 - Wood,C.D., Nisihigaki,T., Furuta,T., Baba,S.A., Darszon,A. (2005). Real-time analysis of the role of Ca2+ in flagellar movement and motility in single sea urchin sperm.
Journal of Cell Biology,
169 (5),
725-731 .
Revisión
56 - Darszon,A., Wood,C.D., Beltran,C., Sanchez,D., Rodriguez,E., Gorelik,J., Korchev,Y.E., Nishigaki,T. (2004). Measuring ion fluxes in sperm.
Methods in Cell Biology,
74,
545-576.
Artículo
57 - Nishigaki,T., Wood,C.D., Tatsu,Y., Yumoto,N., Furuta,T., Elias,D., Shiba,K., Baba,S.A., Darszon,A. (2004). A sea urchin egg jelly peptide induces a cGMP-mediated decrease in sperm intracellular Ca(2+) before its increase.
Developmental Biology,
272 (2),
376-388.
Artículo
58 - Tatsu,Y., Nishigaki,T., Darszon,A., Yumoto,N. (2002). A caged sperm-activating peptide that has a photocleavable protecting group on the backbone amide.
FEBS Letters,
525 (1-3),
20-24.
Revisión
59 - Darszon,A., Beltran,C., Felix,R., Nishigaki,T., Trevino,C.L. (2001). Ion Transport in Sperm Signaling.
Developmental Biology,
240 (1),
1-14.
Artículo
60 - Nishigaki,T., Zamudio,F.Z., Possani,L.D., Darszon,A. (2001). Time-Resolved Sperm Responses to an Egg Peptide Measured by Stopped-Flow Fluorometry.
Biochemical and Biophysical Research Communications,
284 (2),
531-535.
Artículo
61 - Galindo,B.E., Nishigaki,T., Rodriguez,E., Sanchez,D., Beltran,C., Darszon,A. (2000). Speract-receptor interaction and the modulation of ion transport in Strongylocentrotus purpuratus sea urchin sperm.
Zygote,
8 (Suppl 1),
S20-S21.
Artículo; Actas Paper
62 - Hoshi,M., Nishigaki,T., Kawamura,M., Ikeda,M., Gunaratne,J., Ueno,S., Ogiso,M., Moriyama,H., Matsumoto,M. (2000). Acrosome reaction in starfish: signal molecules in the jelly coat and their receptors.
Zygote,
8 (Suppl 1),
S26-S27.
*
Artículo
63 - Nishigaki,T., Chiba,K., Hoshi,M. (2000). A 130-kDa membrane protein of sperm flagella is the receptor for asterosaps, sperm-activating peptides of starfish Asterias amurensis.
Developmental Biology,
219 (1),
154-162.
*
Artículo
64 - Nishigaki,T., Darszon,A. (2000). Real-time measurements of the interactions between fluorescent speract and its sperm receptor.
Developmental Biology,
223 (1),
17-26.
Revisión
65 - Darszon,A., Labarca,P., Nishigaki,T., Espinosa,F. (1999). Ion channels in sperm physiology.
Physiological Reviews,
79 (2),
481-510.
Artículo
66 - Matsumoto,M., Briones,A.V., Nishigaki,T., Hoshi,M. (1999). Sequence analysis of cDNAs encoding precursors of starfish asterosaps.
Developmental Genetics,
25 (2),
130-136.
*
Artículo
67 - Byrne,M., Cerra,A., Nishigaki,T., Hoshi,M. (1997). Infestation of the testes of the Japanese sea star Asterias amurensis by the ciliate Orchitophyra stellarum: A caution against the use of this ciliate for biological control.
Diseases Of Aquatic Organisms,
28 (3),
235-239.
*
Artículo
68 - Nishigaki,T., Chiba,K., Miki,W., Hoshi,M. (1996). Structure and function of asterosaps, sperm-activating peptides from the jelly coat of starfish eggs.
Zygote,
4 (3),
237-245.
*
Revisión
69 - Hoshi,M., Nishigaki,T., Ushiyama,A., Okinaga,T., Chiba,K., Matsumoto,M. (1994). Egg-jelly signal molecules for triggering the acrosome reaction in starfish spermatozoa.
International Journal of Developmental Biology,
38 (2),
167-174.
*
Internacional
70 - Nishigaki,T., Gonzalez-Cota,A.L., Orta Salazar,G.J. (2014). CatSper in Male Infertility. Pathologies of Calcium Channels.
713-728,
Berlin, Heidelberg: Springer.
Internacional
71 - Nishigaki,T., Trevino,C.L. (2012). Study of protein-protein interactions by fluorescence spectroscopy. Tools to Understand Protein-Protein Interactions.
87-100,
Kerala: Research Signpost.
Revisión
72 - Darszon,A., Trevino,C.L., Wood,C., Galindo,B., Rodriguez-Miranda,E., Acevedo,J.J., Hernandez-Gonzalez,E.O., Beltran,C., Martinez-Lopez,P., Nishigaki,T. (2007). Ion channels in sperm motility and capacitation. Society of Reproduction and Fertility supplement.
229-244,
65.
Memoria in extenso
73 - Byrne,M, Cerra,A., Nishigaki,T., Hoshi,M. (1998). Male infertility in Asterias amurensis: a new phenomenon resulting from introduction of the parasitic ciliate Orchitophyra stellarum to Japan. IX International Echiderm Conference.
203-207,
San Francisco, California.
*
Preprint
74 - Novero,A.G., Torres-Rodriguez,P., de la Vega-Beltran,J.L., Schiavi-Ehrenhaus,L.J., Luque,G.M., Carruba,M., Stival,C., Gentile,I., Ritagliati,C., Santi,C.M., Nishigaki,T., Krapf,D., Buffone,M.G., Darszon,A., Trevino,C.L., Krapf,D. (2024). The sodium-proton exchangers sNHE and NHE1 control plasma membrane hyperpolarization in mouse sperm.
bioRxiv,
Preprint posted March 6.
Preprint
75 - Garza-Lopez,E., Aldana,A., Darszon,A., Nishigaki,T., Lopez-Gonzalez,I. (2019). CaV3.1 channel pore pseudo-symmetry revealed by selectivity filter mutations in their domains I/II.
bioRxiv,
Posted May 02, 2019,
626069.
Preprint
76 - Priego-Espinosa,D.A., Darszon,A., Guerrero,A., Gonzalez-Cota,A.L., Nishigaki,T., Martinez-Mekler,G., Carneiro,J. (2018). Modular mathematical analysis of the control of flagellar Ca 2+ -spike trains produced by CatSper and Ca v channels in sea urchin sperm.
bioRxiv,
preprint posted October 18, 2018.
Artículo
77 - Arevalo-Salina,E.L., Nishigaki,T., Olvera,L., Gonzalez-Andrade,M., Xolalpa-Villanueva,W., Lopez-Romero,E.O., Soberon,X., Saab-Rincon,G.. Change in selectivity of estrogen receptor alpha ligand-binding domain by mutations at residues H524/L525.
Biochimica et Biophysica Acta.General Subjects,
1869 (4),
130775.
Artículo
78 - Lopez-Romero,E.O., Arevalo-Salina,E.L., Arcos-Hernandez,C., Sanchez-Guevara,Y., Beltran,C., Saab-Rincon,G., Nishigaki,T.. A FRET-based competitive binding assay using coumestrol and the ligand-binding domain of human estrogen receptor alpha tagged with mTurquoise2 efficiently expressed in E. coli with ethanol.
Protein Expression and Purification,
229,
106667.
Revisión
79 - Chavez,J.C., Carrasquel-Martinez,G., Hernandez-Garduno,S., Matamoros-Volante,A., Trevino,C.L., Nishigaki,T., Darszon,A.. Cytosolic and Acrosomal pH Regulation in Mammalian Sperm.
Cells,
13 (10),
865.
Artículo
80 - Novero,A.G., Torres-Rodriguez,P., de la Vega-Beltran,J.L., Schiavi-Ehrenhaus,L.J., Luque,G.M., Carruba,M., Stival,C., Gentile,I., Ritagliati,C., Santi,C.M., Nishigaki,T., Krapf,D., Buffone,M.G., Darszon,A., Trevino,C.L., Krapf,D.. The sodium-proton exchangers sNHE and NHE1 control plasma membrane hyperpolarization in mouse sperm.
Journal of Biological Chemistry,
300 (12),
107932.
Artículo
81 - Pacheco-Castillo,H., Zagal-Huerta,E.E., Acevedo-Fernandez,J.J., Negrete-Leon,E., Nishigaki,T., Beltran,C.. Hyperglycemia adversely affects critical physiological events related to rat sperm capacitation.
Biochemical and Biophysical Research Communications,
734,
150610.
Artículo
82 - Sanchez-Guevara,Y., Oliver,E.I., Nishigaki,T.. Ca2+ concentrations in mouse sperm mitochondria fluctuate according to the cytosol.
Reproduction,
167 (2),
e230237.
Preprint
83 - Novero,A.G., Torres-Rodriguez,P., de la Vega-Beltran,J.L., Schiavi-Ehrenhaus,L.J., Luque,G.M., Carruba,M., Stival,C., Gentile,I., Ritagliati,C., Santi,C.M., Nishigaki,T., Krapf,D., Buffone,M.G., Darszon,A., Trevino,C.L., Krapf,D.. The sodium-proton exchangers sNHE and NHE1 control plasma membrane hyperpolarization in mouse sperm.
bioRxiv,
Preprint posted March 6.
Artículo
84 - Arcos-Hernandez,C., Suarez-Delgado,E., Islas,L.D., Romero,F., Lopez-Gonzalez,I., Ai,H.W., Nishigaki,T.. How to study a highly toxic protein to bacteria: A case of voltage sensor domain of mouse sperm-specific sodium/proton exchanger.
Protein Expression and Purification,
201,
106172.
Revisión
85 - Arcos-Hernandez,C., Nishigaki,T.. Ion currents through the voltage sensor domain of distinct families of proteins.
Journal of Biological Physics,
49,
393-413.
Artículo
Artículo
87 - Chavez,J.C., Darszon,A., Trevino,C.L., Nishigaki,T.. Quantitative intracellular pH determinations in single live mammalian spermatozoa using the ratiometric dye SNARF-5F.
Frontiers in Cell and Developmental Biology,
7,
366.
Revisión
88 - Darszon,A., Nishigaki,T., Lopez-Gonzalez,I., Visconti,P.E., Trevino,C.L.. Differences and Similarities: The Richness of Comparative Sperm Physiology.
Physiology (Bethesda.),
35 (3),
196-208.
Artículo
89 - De La Rosa,J.A.M., Garcia-Castaneda,M., Nishigaki,T., Gomora,J.C., Mancilla-Percino,T., Avila,G.. Interaction of MDIMP with the voltage-gated calcium channels.
Molecular Pharmacology,
98 (3),
211-221.
Artículo
90 - Garza-Lopez,E., Aldana,A., Darszon,A., Nishigaki,T., Lopez-Gonzalez,I.. CaV3.1 channel pore pseudo-symmetry revealed by selectivity filter mutations in its domains I/II.
Cell Calcium,
89,
102214.
Artículo
91 - Priego-Espinosa,D.A., Darszon,A., Guerrero,A., Gonzalez-Cota,A.L., Nishigaki,T., Martinez-Mekler,G., Carneiro,J.. Modular analysis of the control of flagellar Ca2+-spike trains produced by CatSper and CaV channels in sea urchin sperm.
PLoS Computational Biology,
16 (3),
e1007605.
Artículo
92 - Kinoshita-Terauchi, N., Shiba, K., Terauchi, M., Romero, F., Ramirez-Gomez, H. V., Yoshida, M., Motomura, T., Kawai, H., Nishigaki, T.. High potassium seawater inhibits ascidian sperm chemotaxis, but does not affect the male gamete chemotaxis of a brown alga.
Zygote,
27 (4),
225-231.
Artículo
93 - Romero,F., Nishigaki,T.. Comparative genomic analysis suggests that the sperm-specific sodium/proton exchanger and soluble adenylyl cyclase are key regulators of CatSper among the Metazoa.
Zoological Letters,
5,
25.
Preprint
94 - Garza-Lopez,E., Aldana,A., Darszon,A., Nishigaki,T., Lopez-Gonzalez,I.. CaV3.1 channel pore pseudo-symmetry revealed by selectivity filter mutations in their domains I/II.
bioRxiv,
Posted May 02, 2019,
626069.
Artículo
95 - Chavez, J.C., De la Vega-Beltran JL, Jose, O., Torres, P., Nishigaki, T., Trevino, C.L., Darszon, A.. Acrosomal alkalization triggers Ca2+ release and acrosome reaction in mammalian spermatozoa.
Journal of Cellular Physiology,
233 (6),
4735-4747.
Artículo
96 - Santana-Calvo, C., Romero, F., Lopez-Gonzalez, I., Nishigaki, T.. Robust evaluation of intermolecular FRET using a large Stokes shift fluorophore as a donor.
Biotechniques,
65 (4),
211-218.
Preprint
97 - Priego-Espinosa,D.A., Darszon,A., Guerrero,A., Gonzalez-Cota,A.L., Nishigaki,T., Martinez-Mekler,G., Carneiro,J.. Modular mathematical analysis of the control of flagellar Ca 2+ -spike trains produced by CatSper and Ca v channels in sea urchin sperm.
bioRxiv,
preprint posted October 18, 2018.
Artículo
98 - Romero, F., Santana-Calvo, C., Sanchez-Guevara, Y., Nishigaki, T.. FRET-based binding assay between a fluorescent cAMP analogue and a cyclic nucleotide-binding domain tagged with a CFP.
FEBS Letters,
591 (18),
2869-2878.
Artículo
99 - Arcos-Hernandez,C., Romero,F., Sanchez-Guevara,Y., Beltran,C., Nishigaki,T.. FRET analysis using sperm-activating peptides tagged with fluorescent proteins reveals that ligand-binding sites exist as clusters.
Journal of Experimental Biology,
219 (Pt 4),
508-515.
Artículo
100 - Garza-Lopez,E., Chavez,J.C., Santana-Calvo,C., Lopez-Gonzalez,I., Nishigaki,T.. Cd2+ sensitivity and permeability of a low voltage-activated Ca2+ channel with CatSper-like selectivity filter.
Cell Calcium,
60 (1),
41-50.
Artículo
101 - Gijsbers,A., Nishigaki,T., Sanchez-Puig,N.. Fluorescence Anisotropy as a Tool to Study Protein-protein Interactions.
Journal of Visualized Experiments JoVE,
116.
Artículo
102 - Nishigaki,T., Romero,F., Sanchez-Guevara,Y.. CatSper, el canal de Ca2+ que regula el batido flagelar del espermatozoide en eucariontes.
Revista Iberoamericana de Ciencias,
3 (3),
28-36.
Artículo
103 - Garza-Lopez,E., Sanchez-Carranza,O., Nishigaki,T., Lopez-Gonzalez,I.. Pharmacological identification of endogenous slo1 channel-á1 subunit complexes in CHO cells using three àKTx1 subfamily toxins.
International Journal of Pharmacy & Therapeutics,
6 (1),
11-24.
Revisión
104 - Beltran,C., Rodriguez-Miranda,E., Granados-Gonzalez,G., de De la Torre LG, Nishigaki,T., Darszon,A.. Zn induces hyperpolarization by activation of a K channel and increases intracellular Ca and pH in sea urchin spermatozoa.
Developmental Biology,
394 (1),
15-23.
Revisión
105 - Nishigaki,T., Jose,O., Gonzalez-Cota,A.L., Romero,F., Trevino,C.L., Darszon,A.. Intracellular pH in Sperm Physiology.
Biochemical and Biophysical Research Communications,
450 (3),
1149-1158.
Artículo
106 - Sanchez-Tusie,A.A., Vasudevan,S.R., Churchill,G.C., Nishigaki,T., Trevino,C.L.. Characterization of NAADP-Mediated Calcium Signaling in Human Spermatozoa.
Biochemical and Biophysical Research Communications,
443 (2),
531-536.
Internacional
107 - Nishigaki,T., Gonzalez-Cota,A.L., Orta Salazar,G.J.. CatSper in Male Infertility. Pathologies of Calcium Channels.
713-728,
Berlin, Heidelberg: Springer.
Artículo
108 - Servin-Vences,M.R., Tatsu,Y., Ando,H., Guerrero,A., Yumoto,N., Darszon,A., Nishigaki,T.. A caged progesterone analog alters intracellular Ca2+ and flagellar bending in human sperm.
Reproduction,
144 (1),
101-109.
Internacional
109 - Nishigaki,T., Trevino,C.L.. Study of protein-protein interactions by fluorescence spectroscopy. Tools to Understand Protein-Protein Interactions.
87-100,
Kerala: Research Signpost.
Artículo
110 - Chavez,J.C., de Blas,G.A., de la Vega-Beltran,J.L., Nishigaki,T., Chirinos,M., Gonzalez-Gonzalez,M.E., Larrea,F., Solis,A., Darszon,A., Trevino,C.L.. The opening of maitotoxin-sensitive calcium channels induces the acrosome reaction in human spermatozoa: differences from the zona pellucida.
Asian Journal of Andrology,
13,
159-165.
Revisión
111 - Darszon,A., Nishigaki,T., Beltran,C., Trevino,C.L.. Calcium Channels in the Development, Maturation, and Function of Spermatozoa.
Physiological Reviews,
91 (4),
1305-1355.
Artículo
112 - Guerrero,A., Wood,C.D., Nishigaki,T., Carneiro,J., Darszon,A.. Tuning sperm chemotaxis.
Biochemical Society Transactions,
38 (5),
1270-1274.
Artículo
113 - Guerrero,A., Nishigaki,T., Carneiro,J., Tatsu,Y., Wood,C.D., Darszon,A.. Tuning sperm chemotaxis by calcium burst timing.
Developmental Biology,
344 (1),
52-65.
Artículo
114 - Darszon,A., Guerrero,A., Galindo,B.E., Nishigaki,T., Wood,C.D.. Sperm-activating peptides in the regulation of ion fluxes, signal transduction and motility.
International Journal of Developmental Biology,
52 (5-6),
595-606.
Artículo
115 - de la Sancha,C.U., Martinez-Cadena,G., Lopez-Godinez,J., Castellano,L.E., Nishigaki,T., Darszon,A., Garcia-Soto,J.. Rho-kinase (ROCK) in sea urchin sperm: Its role in regulating the intracellular pH during the acrosome reaction.
Biochemical and Biophysical Research Communications,
364 (3),
470-475 [Corrigendum en Volume 366, Issue 2, 2008, Page 604].
Artículo
116 - Rangel-Mata,F., Mendez-Marquez,R., Martinez-Cadena,G., Lopez-Godinez,J., Nishigaki,T., Darszon,A., Garcia-Soto,J.. Rho, Rho-kinase, and the actin cytoskeleton regulate the Na(+)-H(+) exchanger in sea urchin eggs.
Biochemical and Biophysical Research Communications,
352 (1),
264-269.
Artículo
117 - Wood,C.D., Nishigaki,T., Tatsu,Y., Yumoto,N., Baba,S.A., Whitaker,M., Darszon,A.. Altering the speract-induced ion permeability changes that generate flagellar Ca(2+) spikes regulates their kinetics and sea urchin sperm motility.
Developmental Biology,
306 (2),
525-537.
Revisión
118 - Darszon,A., Trevino,C.L., Wood,C., Galindo,B., Rodriguez-Miranda,E., Acevedo,J.J., Hernandez-Gonzalez,E.O., Beltran,C., Martinez-Lopez,P., Nishigaki,T.. Ion channels in sperm motility and capacitation. Society of Reproduction and Fertility supplement.
229-244,
65.
Revisión
119 - Darszon,A., Acevedo,J.J., Galindo,B.E., Hernandez-Gonzalez,E.O., Nishigaki,T., Trevino,C.L., Wood,C., Beltran,C.. Sperm channel diversity and functional multiplicity.
Reproduction,
131 (6),
977-988.
Artículo
120 - Nishigaki,T., Wood,C.D., Shiba,K., Baba,S.A., Darszon,A.. Stroboscopic illumination using light-emitting diodes reduces phototoxicity in fluorescence cell imaging.
Biotechniques,
41 (2),
191-197.
Artículo
121 - Trevino,C.L., de la Vega-Beltran,J.L., Nishigaki,T., Felix,R., Darszon,A.. Maitotoxin potently promotes Ca(2+) influx in mouse spermatogenic cells and sperm, and induces the acrosome reaction.
Journal of Cellular Physiology,
206 (2),
449-456.
Revisión
122 - Darszon,A., Nishigaki,T., Wood,C., Trevino,C.L., Felix,R., Beltran,C.. Calcium channels and ca(2+) fluctuations in sperm physiology.
International Review of Cytology,
243,
79-172.
Artículo
123 - Shiba,K., Ohmuro,J., Mogami,Y., Nishigaki,T., Wood,C.D., Darszon,A., Tatsu,Y., Yumoto,N., Baba,S.A.. Sperm-activating Peptide induces asymmetric flagellar bending in sea urchin sperm.
Zoological Science,
22 (3),
293-299.
Artículo
124 - Wood,C.D., Nisihigaki,T., Furuta,T., Baba,S.A., Darszon,A.. Real-time analysis of the role of Ca2+ in flagellar movement and motility in single sea urchin sperm.
Journal of Cell Biology,
169 (5),
725-731 .
Revisión
125 - Darszon,A., Wood,C.D., Beltran,C., Sanchez,D., Rodriguez,E., Gorelik,J., Korchev,Y.E., Nishigaki,T.. Measuring ion fluxes in sperm.
Methods in Cell Biology,
74,
545-576.
Artículo
126 - Nishigaki,T., Wood,C.D., Tatsu,Y., Yumoto,N., Furuta,T., Elias,D., Shiba,K., Baba,S.A., Darszon,A.. A sea urchin egg jelly peptide induces a cGMP-mediated decrease in sperm intracellular Ca(2+) before its increase.
Developmental Biology,
272 (2),
376-388.
Artículo
127 - Tatsu,Y., Nishigaki,T., Darszon,A., Yumoto,N.. A caged sperm-activating peptide that has a photocleavable protecting group on the backbone amide.
FEBS Letters,
525 (1-3),
20-24.
Revisión
128 - Darszon,A., Beltran,C., Felix,R., Nishigaki,T., Trevino,C.L.. Ion Transport in Sperm Signaling.
Developmental Biology,
240 (1),
1-14.
Artículo
129 - Nishigaki,T., Zamudio,F.Z., Possani,L.D., Darszon,A.. Time-Resolved Sperm Responses to an Egg Peptide Measured by Stopped-Flow Fluorometry.
Biochemical and Biophysical Research Communications,
284 (2),
531-535.
Artículo
130 - Galindo,B.E., Nishigaki,T., Rodriguez,E., Sanchez,D., Beltran,C., Darszon,A.. Speract-receptor interaction and the modulation of ion transport in Strongylocentrotus purpuratus sea urchin sperm.
Zygote,
8 (Suppl 1),
S20-S21.
Artículo; Actas Paper
131 - Hoshi,M., Nishigaki,T., Kawamura,M., Ikeda,M., Gunaratne,J., Ueno,S., Ogiso,M., Moriyama,H., Matsumoto,M.. Acrosome reaction in starfish: signal molecules in the jelly coat and their receptors.
Zygote,
8 (Suppl 1),
S26-S27.
*
Artículo
132 - Nishigaki,T., Chiba,K., Hoshi,M.. A 130-kDa membrane protein of sperm flagella is the receptor for asterosaps, sperm-activating peptides of starfish Asterias amurensis.
Developmental Biology,
219 (1),
154-162.
*
Artículo
133 - Nishigaki,T., Darszon,A.. Real-time measurements of the interactions between fluorescent speract and its sperm receptor.
Developmental Biology,
223 (1),
17-26.
Revisión
134 - Darszon,A., Labarca,P., Nishigaki,T., Espinosa,F.. Ion channels in sperm physiology.
Physiological Reviews,
79 (2),
481-510.
Artículo
135 - Matsumoto,M., Briones,A.V., Nishigaki,T., Hoshi,M.. Sequence analysis of cDNAs encoding precursors of starfish asterosaps.
Developmental Genetics,
25 (2),
130-136.
*
Memoria in extenso
136 - Byrne,M, Cerra,A., Nishigaki,T., Hoshi,M.. Male infertility in Asterias amurensis: a new phenomenon resulting from introduction of the parasitic ciliate Orchitophyra stellarum to Japan. IX International Echiderm Conference.
203-207,
San Francisco, California.
*
Artículo
137 - Byrne,M., Cerra,A., Nishigaki,T., Hoshi,M.. Infestation of the testes of the Japanese sea star Asterias amurensis by the ciliate Orchitophyra stellarum: A caution against the use of this ciliate for biological control.
Diseases Of Aquatic Organisms,
28 (3),
235-239.
*
Artículo
138 - Nishigaki,T., Chiba,K., Miki,W., Hoshi,M.. Structure and function of asterosaps, sperm-activating peptides from the jelly coat of starfish eggs.
Zygote,
4 (3),
237-245.
*
Revisión
139 - Hoshi,M., Nishigaki,T., Ushiyama,A., Okinaga,T., Chiba,K., Matsumoto,M.. Egg-jelly signal molecules for triggering the acrosome reaction in starfish spermatozoa.
International Journal of Developmental Biology,
38 (2),
167-174.
*
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