Instituto de Biotecnologia UNAM

Grupo del Dr. Takuya Nishigaki

Study of the sperm motility regulation using optical too

Study of the sperm motility regulation using optical too

Spermatozoa are male gametes, which are generally small and do not have an activity of transcription either translation; it means they are genetically silent. However, they surprisingly have great capacity to respond to several physicochemical stimuli and complete its objective: find the oocyte and fertilize it. One of the most important functions of spermatozoa is the motility propelled by its flagellum. In fact, any defects in flagellar function can lead to a male infertility

We are interested in the regulation of sperm motility, in which Ca2+, cAMP, pH and ATP/ADP ratio are known to be crucial intracellular factors. One of our aims is to determine the correlation of these factor dynamics and the flagellar beating patterns in vitro (eventually in vivo) and to elucidate how these factors are regulated and how they act on the flagellum.

Considerando las limitaciones que presenta el espermatozoide, no se pueden aplicar los métodos convencionales de biología celular tales como cultivo celular y transfección. Por lo anterior hemos implementado una serie de herramientas alternativas que nos permitirán estudiar este modelo biológico como son:
Since spermatozoa have unique characteristics as mentioned above, conventional methods for cell biology, such as cell culture and transfection using expression vectors or iRNA, are not applicable. In order to complete our objectives, we have to combine several experimental techniques as listed below:

  1. Spectrofluorometry using membrane-permeable fluorescent indicators by Ca2+, pHi, Na+, etc.
  2. Fluorescence imaging of motile sperm by stroboscopic illumination.
  3. Compuestos fotoactivables de distintos agonistas de la motilidad flagelar para inducir un efecto en el espermatozoide sin generar perturbaciones mecánicas.
  4. Caged compounds (to trigger sperm response quickly without mechanical perturbation).
  5. Protein engineering to study the molecular interaction between cAMP and its target.
  6. Protein engineering to develop new fluorescent indicators for cAMP.
  7. Transgenic mice that express a fluorescent indicator in the mature sperm.

Dr. Takuya Nishigaki
Investigador
Tutor de Maestría y Doctorado
Dr. Ignacio Lopez
Investigador
Tutor de Maestría y Doctorado
M.C. Yoloxochitl Sanchez
Técnico Académico
Bryan Alexis Alvarado
Estudiante
Biol. César Arcos
Estudiante
Ing. Andrea Zacnite Cuevas
Estudiante
Valery Itzxchel Escobedo
Estudiante
M.C. Sandra Hernández
Estudiante
Columba Alejandra López
Estudiante
M.C. Edith Odemaris López
Estudiante
Miguel A. Trujillo
Auxiliar de Laboratorio


UNAM | UNAM
Morelos
| Site Map | Virtual Biotechnology Library | About us | Services | email | Publications
Graduate Studies | IBT Seminars | Personnel | Library | Books and on line documents | Localization | SiBioTec | Main Page
972 : 10240