Instituto de Biotecnologia UNAM

IBT-UNAM : Dr. Takuya Nishigaki Shimizu

Dr.

Dr. Takuya Nishigaki Shimizu


Líder Académico »» Grupo

Investigador

Tutor de Maestría y Doctorado

Nivel II del SNI

Departamento de Genética del Desarrollo y Fisiología Molecular

Líneas de investigación

email: takuya@ibt.unam.mx

Teléfonos

Oficina +52 (777) 329-1709
desde la CDMX 562-27709

red UNAM 27709

Laboratorio +52 (777) 329-1777 ext 38133.
desde la CDMX 562-27777 ext 38133

red UNAM 38133


- Licenciatura: Ciencias, Instituto Tecnologico de Tokyo (1992)
- Maestría: en Biociencias, Instituto Tecnologico de Tokyo (1994)
- Doctorado: en Biociencias, Instituto Tecnologico de Tokyo (1997)
- Estancia de investigacion en el laboratorio del Dr. Alberto Darszon, del IBt-UNAM (1997-1999)


Estudiantes

Columba Alejandra López

Biol. César Arcos

M.C. Sandra Hernández

M.C. Edith Odemaris López

Publicaciones recientes

De La Rosa,J.A.M. GarcIa-Castaneda,M. Nishigaki,T. Gomora,J.C. Mancilla-Percino,T. Avila,G. 2020. Interaction of MDIMP with the voltage-gated calcium channels Molecular Pharmacology, Jun 25 [Epub ahead of print], .

Garza-Lopez,E. Aldana,A. Darszon,A. Nishigaki,T. Lopez-Gonzalez,I. 2020. CaV3.1 channel pore pseudo-symmetry revealed by selectivity filter mutations in its domains I/II Cell Calcium, 89, 102214.

Darszon,A. Nishigaki,T. Lopez-Gonzalez,I. Visconti,P.E. Trevino,C.L. 2020. Differences and Similarities: The Richness of Comparative Sperm Physiology Physiology (Bethesda.), 35, 196-208.

Priego-Espinosa,D.A. Darszon,A. Guerrero,A. Gonzalez-Cota,A.L. Nishigaki,T. Martinez-Mekler,G. Carneiro,J. 2020. Modular analysis of the control of flagellar Ca2+-spike trains produced by CatSper and CaV channels in sea urchin sperm PLoS Computational Biology, 16, e1007605.

Chavez,J.C. Darszon,A. Trevino,C.L. Nishigaki,T. 2020. Quantitative intracellular pH determinations in single live mammalian spermatozoa using the ratiometric dye SNARF-5F Frontiers in Cell and Developmental Biology, 7, 366.

Romero,F. Nishigaki,T. 2019. Comparative genomic analysis suggests that the sperm-specific sodium/proton exchanger and soluble adenylyl cyclase are key regulators of CatSper among the Metazoa Zoological Letters, 5, 25.

Kinoshita-Terauchi,N. Shiba,K. Terauchi,M. Romero,F. Ramirez-Gomez,H.V. Yoshida,M. Motomura,T. Kawai,H. Nishigaki,T. 2019. High potassium seawater inhibits ascidian sperm chemotaxis, but does not affect the male gamete chemotaxis of a brown alga Zygote, 27, 225-231.

Santana-Calvo,C. Romero,F. Lopez-Gonzalez,I. Nishigaki,T. 2018. Robust evaluation of intermolecular FRET using a large Stokes shift fluorophore as a donor Biotechniques, 65, 211-218.

Chavez,J.C. De la Vega-Beltran JL Jose,O. Torres,P. Nishigaki,T. Trevino,C.L. Darszon,A. 2018. Acrosomal alkalization triggers Ca2+ release and acrosome reaction in mammalian spermatozoa Journal of Cellular Physiology, 233, 4735-4747.

Romero,F. Santana-Calvo,C. Sanchez-Guevara,Y. Nishigaki,T. 2017. FRET-based binding assay between a fluorescent cAMP analogue and a cyclic nucleotide-binding domain tagged with a CFP FEBS Letters, 591, 2869-2878.

Nishigaki,T. Romero,F. Sanchez-Guevara,Y. 2016. CatSper, el canal de Ca2+ que regula el batido flagelar del espermatozoide en eucariontes Revista Iberoamericana de Ciencias, 3, 28-36.

Gijsbers,A. Nishigaki,T. Sanchez-Puig,N. 2016. Fluorescence Anisotropy as a Tool to Study Protein-protein Interactions Journal of Visualized Experiments JoVE, 116, .

Garza-Lopez,E. Chavez,J.C. Santana-Calvo,C. Lopez-Gonzalez,I. Nishigaki,T. 2016. Cd2+ sensitivity and permeability of a low voltage-activated Ca2+ channel with CatSper-like selectivity filter Cell Calcium, 60, 41-50.

Arcos-Hernandez,C. Romero,F. Sanchez-Guevara,Y. Beltran,C. Nishigaki,T. 2016. FRET analysis using sperm-activating peptides tagged with fluorescent proteins reveals that ligand-binding sites exist as clusters Journal of Experimental Biology, 219, 508-515.


Otras Publicaciones

Garza-Lopez,E. Aldana,A. Darszon,A. Nishigaki,T. Lopez-Gonzalez,I. 2019. CaV3.1 channel pore pseudo-symmetry revealed by selectivity filter mutations in their domains I/II bioRxiv, Preprint posted May 02, 2019, 626069.

Priego-Espinosa,D.A. Darszon,A. Guerrero,A. Gonzalez-Cota,A.L. Nishigaki,T. Martinez-Mekler,G. Carneiro,J. 2018. Modular mathematical analysis of the control of flagellar Ca2+-spike trains produced by CatSper and Cav channels in sea urchin sperm bioRxiv, preprint posted October 18, 2018, .

* Indica publicación con otra institución de adscripción



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