14Abr - 2021
CONFERENCIA MAGISTRAL: La educación en valores para la ciudadanía en los títulos universitarios
12:00 PM - 02:00 PM|Dr. Cruz Pérez Pérez
Seminario
Dr. Cruz Pérez Pérez
Investigador del Departamento de Teoría de la Educación, Universidad de Valencia
Modera:
Dra. María Teresa Yurén Camarena PITC del CIIDU
Actualizado 2021-04-12 08:54:31
Biomolecular condensates in cellular stress responses and disease.
Simon Alberti. Professor of Cellular Biochemistry. Technische Universität Dresden. Center for Molecular and Cellular Bioengineering (CMCB). Biotechnology Center (BIOTEC)
Sede: Auditorio "Dr. Francisco G. Bolívar Zapata" del IBt/UNAM
01-Abril-2024 al 01-Abril-2024
05:00 PM
Dr. Rafael Martinez Gallegos
05:00 PM
Dr. Rafael Martinez Gallegos
Homologous recombination cloning: Tips, tricks and advantages
Using the endogenous homologous recombination of living organisms for cloning (HR-cloning) was first described in yeast and eventually in bacteria. Although not so widespread, this method allows the straightforward engineering of fusion proteins with the ability of exchanging elements from 1 nt to several kb within a plasmid. Therefore it can be used to manipulate codons or transcription factor target sequences as well as to study regulatory regions like full promoters or UTRs. Likewise, reporter markers can be exchanged having either N- or C-terminal variants. It also offers the possibility of engineering customized plasmids providing a different strategy to overcome difficulties in the cloning and editing of challenging DNA fragments. The manipulation steps are minimized and any recA1 bacteria strain is suitable for it. This method has been tested with common and customized plasmids with a range up to 15 kb. Its simplicity allows the implementation in any molecular biology laboratory without the requirement of any commercial Kit. I will discuss examples of HR-cloning on a wide variety of organisms, from plants to Drosophila.